Prevelance of Central Nervous System Cryptococcosis in Human Immunodeficiency Virus Reactive Hospitalised Patients
Authors/Creators
- 1. Assistant Professor, Department of Microbiology, Viswabharathi Medical College and Hospital, Kurnool
- 2. Professor, Department of Microbiology, Viswabharathi Medical College and Hospital, Kurnool
Description
Cryptococcosis is a systemic mycosis caused by two environmental species, Cryptococcus neoformans and Cryptococcus gattii. Cryptococcus neoformans is distributed worldwide and is primarily an opportunistic fungal pathogen, most commonly associated with HIV infection, meningoencephalitis being the most common presentation. Early diagnosis is essential to prevent the complications of Cryptococcosis as it is associated with high mortality and morbidity. Materials & Methods: We did a comparative study on detection of Cryptococcal infection by conventional method and latex agglutination. A total of 71 CSF samples from HIV seropositive cases, with clinical suspicion of Cryptococcal meningitis, were processed by conventional methods – Gram staining, India ink preparation, culture on Sabouraud’s Dextrose agar. Antigen detection was done on all CSF samples using latex agglutination test. Conclusion: The prevalence of Cryptococcal infection was found to be 7.04%. Latex agglutination was more sensitive (100%) than conventional methods (80%).
Abstract (English)
Cryptococcosis is a systemic mycosis caused by two environmental species, Cryptococcus neoformans and Cryptococcus gattii. Cryptococcus neoformans is distributed worldwide and is primarily an opportunistic fungal pathogen, most commonly associated with HIV infection, meningoencephalitis being the most common presentation. Early diagnosis is essential to prevent the complications of Cryptococcosis as it is associated with high mortality and morbidity. Materials & Methods: We did a comparative study on detection of Cryptococcal infection by conventional method and latex agglutination. A total of 71 CSF samples from HIV seropositive cases, with clinical suspicion of Cryptococcal meningitis, were processed by conventional methods – Gram staining, India ink preparation, culture on Sabouraud’s Dextrose agar. Antigen detection was done on all CSF samples using latex agglutination test. Conclusion: The prevalence of Cryptococcal infection was found to be 7.04%. Latex agglutination was more sensitive (100%) than conventional methods (80%).
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IJPCR,Vol15,Issue1,Article125.pdf
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Additional details
Dates
- Accepted
-
2023-01-10
Software
- Repository URL
- https://impactfactor.org/PDF/IJPCR/15/IJPCR,Vol15,Issue1,Article125.pdf
- Development Status
- Active
References
- 1. Speed B, Dunt D. Clinical and host differences between infections with the two varieties of Cryptococcus neoformans. Clin Infect Dis. 1995;21(1):28–36. 2. Wallace RS, Meyer W, Wanke B, Costa SPSE, Trilles L, Nascimento JLM, et al. Primary endemic Cryptococcosis gattii by molecular type VGII in the state of Pará, Brazil. Mem Inst Oswaldo Cruz. 2008;103(8):813–818. 3. Jain N, Wickes BL, Keller SM, Fu J, Casadevall A, Jain P, et al. Molecular Epidemiology of Clinical Cryptococcus neoformans Strains from India. J Clin Microbiol. 2005;43(11):5733–5742. 4. Prado M, Da Silva MB, Laurenti R, Travassos L, Taborda C. Mortality due to systemic mycoses as a primary cause of death or in association with AIDS in Brazil: a review from 1996 to 2006. Mem Inst Oswaldo Cruz. 2009;104(3):513– 521. 5. Lindenberg A, De SC, Chang MR, Paniago AMM, Lazéra M, Dos S, Moncada PMF, et al. Clinical and epidemiological features of 123 cases of cryptococcosis in Mato Grosso do Sul, Brazil. Rev Inst Med trop S Paulo. 2008;50(2):75–78. 6. Prasad KN, Argawal J, Nag VL, Verma AK, Dixit AK, Ayyagari A. Cryptococcal infection in patients with clinically diagnosed meningitis in a tertiary care center. Neurol India. 2003;51(3):364– 366. 7. Kumar S, Wanchu A, Chakrabarti A, Sharma A, Bambery P, et al. Cryptococcal meningitis in HIV infected experience from a North Indian tertiary center. Neurol India. 2008; 56:444–449. 8. Tay ST, Rohani MY, Soo Hoo TS, Hamimah H. Epidemiology of cryptococcosis in Malaysia. Mycoses. 2009; 53:509–514. 9. Tintelnot K, Lemmer K, Losert H, Schar G, Polak A. Follow-up of epidemiological data of cryptococcosis in Austria, Germany and Switzerland with special focus on the characterization ofclinical isolates. Mycoses. 2004; 47:455– 464. 10. Chen S, Sorrell T, Nimmo G, Speed B, Currie B, Ellis D, et al. Epidemiology and host and variety-dependent characterization of infection due to Cryptococcus neoformans in Australia and New Zealand. Clin Infect Dis. 2000; 31:499–508. 11. Mbanya DN, Zebaze R, Minkoulou EM, Binam F, Koulla S, Obounou A. Clinical and epidemiologic trends in HIV/AIDS Patients in a hospital setting of Yaounde, Cameroon: a 6-year perspective. Int J Infect Dis. 2002; 6:134–138. 12. Sasisopin K, Sirirat W, Roongnapa P, Somnuek S. Cryptococcosis in human immunodeûciency virus-negative patients. Int J Infect Dis. 2006; 10:72–78. 13. Thakur R, Sarma S, Kushwaha S. Prevalence of HIV-associated cryptococcal meningitis and utility of microbiological determinants for its diagnosis in a tertiary care center. Indian J Path Microbiol. 2008;51(2):212–214. 14. Taneji J, Mishra B, Bhargava A, Loomba P, Dogra V, Thakur A. Cryptococcal meningitis in a tertiary care hospital. Jpn J Med Mycol. 2009; 50:95–99. 15. Kisenge PR, Hawkins AT, Maro V P, Mchele JPD, Swai NS, Mueller A, Houpt ER. Low CD4 count plus coma predicts cryptococcal meningitis in Tanzania. BMC Infect Dis. 2007; 7:39. 16. Centers for Disease Control and Prevention, author. Revised classification system for HIV infection and expanded surveillance case definiton for AIDS among adolescents and adults. MMWR (Morb Mortal Wkl Rep) 1992;41(RR17):1–19. 17. Park BJ, Wannemuehler KA, Marstonc BJ, Govender N, Pappas PG, Chiller TM. Estimation of the current global burden of cryptococcal meningitis among persons living with HIV/AIDS. AIDS. 2009;23(4):531–532. 18. Antinori S, Ridolfo A, Fasan M, Magni C, Galimberti L, Milazzo L, Sollima S, et al. AIDS-associated cryptococcosis: a comparison of epidemiology, clinical features and outcome in the pre- and postHAART eras. Experience of a single centre in Italy. HIV Med. 2009; 10:6–11. 19. Ubonvan J, Somnuek S, Sasisopin K. Comparison of clinical features and survival between cryptococcosis in Human Immunodeficiency Virus (HIV)- positive and HIV-negative patients. Jpn J Infect Dis. 2008; 61:111–115. 20. Baradkar V, Mathur M, De A, Kumar S, Rathi M. Prevalence and clinical presentation of cryptococcal meningitis among HIV seropositive patients. Indian J Sex Transm Dis & AIDS. 2009;30(1):19–22 21. Bogaerts J, Roubroy D, Taelman H, Kagame A, Aziz MA, Swinne D, et al. AIDS-associated cryptococcal meningitis in Rwanda (1983–1992). epidemiologic and diagnostic features. J Infect. 1999; 39:32–37. 22. Gumbo T, Kadzirange G, Mielke J, Gangaidzo IT, Hakim JG. Cryptococcus neoformans meningoencephalitis in African children with acquired immunodeficiency syndrome. Pediatr Infect Dis J. 2002; 21:54–56 23. Pastagia M, Caplivski D. Disseminated cryptococcosis resulting in miscarriage in a woman without other immunocompromise: a case report. Int J Infect Dis. 2010;14: e441–e443. 24. Lohoue PJ, Same EA, Nomo OSA. Cryptococcose et syndrome de l'immunodéficience acquise à Yaoundé Méd Mal Infect. 1992;22(1):30–31.