A Bacteriological Study of Various Culture Samples among Patients Attending Tertiary Care Hospital, Morbi, Gujarat
Creators
- 1. Senior Resident Doctor, Dept. of Community Medicine GMERS Medical College, Morbi (Guj), India
- 2. Resident Doctor, Department of Anaesthesia, NHL Municipal Medical College, Ahmedabad (Guj), India
- 3. Assistant Professor, Department of Microbiology, Zydus Medical College and hospital, Dahod (Guj), India
- 4. Assistant Professor, Department of Microbiology, GMERS Medical College, Morbi (Guj), India
Description
Background: India face significant risks from infections with multi-drug resistant (MDR) bacteria, which are linked to antibiotic overuse and misuse. The antibiogram profiles of bacteria isolated from infections in patients of tertiary care hospital Morbi were determined in this study. Methods: A hospital-based cross-sectional study was done between February 2024 and June 2024. Various clinical specimens were sampled from patients and analysed for aerobic bacterial isolation and Kirby–Bauer disk diffusion susceptibility testing. Results: From the 239 clinical specimens processed, 61 (25.5%) were culture-positive for aerobic bacterial pathogens. Culture-confirmed positivity was higher in urine (29.03%), pus (28.95%) and blood (24.39%) samples. Escherichia coli 18 (29.51%) and 16 (26.23%) of Klebsiella pneumoniae from Gram-negative bacteria were the predominant bacterial isolates, while Staphylococcus aureus 12 (19.67%) and Staphylococcus sp. 3(4.92%) were from Gram-positive bacteria. Overall, 72.1% of the isolates were found to be MDR. The proportion of MDR among Escherichia coli, Staphylococcus sp., Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa was 100%, 1005, 93.755, 50% and 33.3% respectively. Gram-positive bacteria demonstrated resistance rates of 58.3%, 40%, 37.5%, 35.7% and 29.4% for cefoxitin, gentamicin, azithromycin, ciprofloxacin, erythromycin, respectively. While Gram-negative demonstrated resistance rates of 85.7%, 75%, 69.7%, 66.7%, 65.9%, 61.8%, 61.1%, 60.5% were recorded for ampicillin cefotaxime, ceftriaxone, cefoperazone piperacillin/tazobactam, ceftazidime, ampicillin/sulbactam, ciprofloxacin respectively. Conclusion: The main problem is infections caused by bacterial isolates, resistant to most antibiotics. With an alarmingly high number of MDR isolates, the majority of the bacteria that were found to be resistant to the commonly used medicines. Antimicrobial susceptibility testing should therefore be used as a guide for treatment decisions and physicians should use reason when selecting medicines.
Abstract (English)
Background: India face significant risks from infections with multi-drug resistant (MDR) bacteria, which are linked to antibiotic overuse and misuse. The antibiogram profiles of bacteria isolated from infections in patients of tertiary care hospital Morbi were determined in this study. Methods: A hospital-based cross-sectional study was done between February 2024 and June 2024. Various clinical specimens were sampled from patients and analysed for aerobic bacterial isolation and Kirby–Bauer disk diffusion susceptibility testing. Results: From the 239 clinical specimens processed, 61 (25.5%) were culture-positive for aerobic bacterial pathogens. Culture-confirmed positivity was higher in urine (29.03%), pus (28.95%) and blood (24.39%) samples. Escherichia coli 18 (29.51%) and 16 (26.23%) of Klebsiella pneumoniae from Gram-negative bacteria were the predominant bacterial isolates, while Staphylococcus aureus 12 (19.67%) and Staphylococcus sp. 3(4.92%) were from Gram-positive bacteria. Overall, 72.1% of the isolates were found to be MDR. The proportion of MDR among Escherichia coli, Staphylococcus sp., Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa was 100%, 1005, 93.755, 50% and 33.3% respectively. Gram-positive bacteria demonstrated resistance rates of 58.3%, 40%, 37.5%, 35.7% and 29.4% for cefoxitin, gentamicin, azithromycin, ciprofloxacin, erythromycin, respectively. While Gram-negative demonstrated resistance rates of 85.7%, 75%, 69.7%, 66.7%, 65.9%, 61.8%, 61.1%, 60.5% were recorded for ampicillin cefotaxime, ceftriaxone, cefoperazone piperacillin/tazobactam, ceftazidime, ampicillin/sulbactam, ciprofloxacin respectively. Conclusion: The main problem is infections caused by bacterial isolates, resistant to most antibiotics. With an alarmingly high number of MDR isolates, the majority of the bacteria that were found to be resistant to the commonly used medicines. Antimicrobial susceptibility testing should therefore be used as a guide for treatment decisions and physicians should use reason when selecting medicines.
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IJPCR,Vol16,Issue7,Article124.pdf
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Additional details
Dates
- Accepted
-
2024-07-17
Software
- Repository URL
- https://impactfactor.org/PDF/IJPCR/16/IJPCR,Vol16,Issue7,Article124.pdf
- Development Status
- Active
References
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