Role of Paclobutrazol on Root Stem and Leaf Inner Structure of Arabidopsis Thaliana L.0 Grown Under Different Light Intensities
- 1. Department of Biology, College of Education for Pure Science, University of Basrah, Basrah, Iraq.
- 1. Department of Biology, College of Education for Pure Science, University of Basrah, Basrah, Iraq.
Description
Abstract: Light is one of the most important environmental factors affecting plant growth and development. Paclobutrazol (PBZ) is one of the members of Triazole compounds that possess the qualities of growth regulators. The study aimed to investigate the possible changes in anatomical structures of the root, stem, and leaf of A. thaliana grown under different light intensities and the role of pbz in these circumstances. The anatomical characteristics of the root, stem, and leaves of A. thaliana col.0 were measured after treating its seedlings at two weeks of age with Hock land's solution at different light intensities (3000, 6000, 9000, 12000 Lux) for four weeks. The results showed changes in anatomical characteristics due to light stress represented by a significant moral decrease in the diameter of the root, vascular cylinder, and thickness of the epidermis and cortex. It also led to a reduction in the width of the stem, the thickness of the xylem, and an increase in the thickness of the epidermis and phloem. Light stress caused a significant decrease in leaf thickness and the ratio of palisade tissue thickness to spongy tissue thickness. They addition the growth regulator PBZ causes a substantial increase in all of the anatomical characteristics of the stem, root, and leaves.
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Additional details
Identifiers
- DOI
- 10.54105/ijab.B1031.04010424
- EISSN
- 2582-9475
Dates
- Accepted
-
2024-04-15Manuscript received on 12 March 2024 | Revised Manuscript received on 12 April 2024 | Manuscript Accepted on 15 April 2024 | Manuscript published on 30 April 2024
References
- Yang F; Feng L; Liu Q; Wu X; Fan Y; Raza MA et al. Effect of interactions between light intensity and red-to-far-red ratio on the photosynthesis of soybean leaves under shade condition. Environ. Exp. Bot. 2018, 150, 79-87. https://doi.org/10.1016/j.envexpbot.2018.03.008
- Zhu H; Li X; Zhai, W; Liu Y; Gao Q; Liu J, et al (2017). Effects of low light on photosynthetic properties, antioxidant enzyme activity, and anthocyanin accumulation in purple pakchoi (Brassica campestris ssp. Chinensis Makino). PLoS ONE12 (6) : 1-17. https://doi.org/10.1371/journal.pone.0179305
- Lichtenthaler, H, Burkart, S. (1999). Photosynthesis and high stress. Bulgarian J. of Plant Physiology, 25(3-4):3-16.
- Boyang J, Tushar K; Amirita S; Sagar D, Khare T (2019). Light Stress Responses and Prospects for Engineering Light Stress Tolerance in Crop Plants. J. of Plant Growth Regulation, 38:1489 – 1506. https://doi.org/10.1007/s00344-019-09951-8
- Fletcher RA, Gilley A, Davis, TD, Sankhla N (2000). Triazoles as plant growth regulators and stress protectants. Horticultural Review, 24, 55- 138 .https://doi.org/10.1002/9780470650776.ch3
- Hajihashemi S, Kiarostami K. Effects of paclobutrazol and salt stress on growth and ionic contents in two cultivars of wheat. Pak J Biol Sci. 2007 Jan 1;10(1):41-8. https://doi.org/10.3923/pjbs.2007.41.48
- Jaleel CA, P. Manivannan, B. Sankar, A. Kishorekumar, S. Sankari, R. Panneerselvam, 2007. Paclobutrazol enhances photosynthesis and ajmalicine production in Catharanthus roseus. Process Biochemistry 42 (2007):1566–1570. https://doi.org/10.1016/j.procbio.2007.08.006
- Kircher S, Schopfer P (2012). Photosynthetic sucrose acts as cotyledon-derived long-distance signal to control root growth during early seedling development in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 109, 11217 –11221. https://doi.org/10.1073/pnas.1203746109
- Yokawa K, Kagenishi T, Kawano T, Mancuso S, Baluška F (2011). Illumination of Arabidopsis roots induces immediate burst of ROS production. Plant Signal. Behav. 6, 1460–1464 https://doi.org/10.4161/psb.6.10.18165
- Bhalerao RP, Eklöf J, Ljung K, Marchant A, Bennett M, Sandberg G (2002). Shoot-derived auxin is essential for early lateral root emergence in Arabidopsis seedlings. Plant J. 29, 325–332 . https://doi.org/10.1046/j.0960-7412.2001.01217.x
- Salisbury FJ, Hall A, Grierson CS, Halliday KJ (2007). Phytochrome coordinates Arabidopsis shoot and root development. Plant J. 50, 429–438. https://doi.org/10.1111/j.1365-313X.2007.03059.x
- Swarup K, Benková E, Swarup R, Casimiro I, Péret B, Yang Y, et al (2008). The auxin influx carrier LAX3 promotes lateral root emergence. Nat. Cell Biol. 10,946–954. https://doi.org/10.1038/ncb1754
- Abdalla N; Taha N; Bayoumi Y; El-Ramady H; Shalaby TA. Paclobutrazol applications in agriculture, plant tissue cultures and its potential as stress ameliorant, A Mini-Review. Environ. Biodivers. Soil Secur. 2021, 5, 245–257. https://doi.org/10.21608/jenvbs.2021.95536.1143
- Detpitthayanan S; Romyanon K; Songnuan W; Metam M; Pichakum A. Paclobutrazol Application Improves Grain 2AP Content of Thai Jasmine Rice KDML105 under Low-Salinity Conditions. J. Crop Sci. Biotechnol. 2019, 22, 275–282. https://doi.org/10.1007/s11738-007-0025-6
- Jaleel CA; Gopi R; Manivannan P; Panneerselvam R. Responses of antioxidant defense system of Catharanthus roseus (L.) G. Don. to paclobutrazol treatment under salinity. Acta Physiol. Plant. 2007, 29, 205–209 .
- Waqas M; Yaning C; Iqbal H; Shareef M; Rehman H; Yang Y. Paclobutrazol improves salt tolerance in quinoa, Beyond the stomatal and biochemical interventions. J. Agron. Crop Sci. 2017, 203, 315–322 https://doi.org/10.1111/jac.12217
- Gao Z; Khalid M; Jan F; Rahman SU; Jiang X; Yu X. Effects of light-regulation and intensity on the growth, physiological and biochemical properties of Aralia elata (Miq.) seedlings. S. Afr. J. Bot. 2019, 121, 456–462. https://doi.org/10.1016/j.sajb.2018.12.008
- Wu YS; Gong WZ; Yang F; Wang XC; Yong TW; Yang WY. Responses to shade and subsequent recovery of soybean in maize-soybean relay strip intercropping. Plant Prod. Sci. 2016, 19, 206–214. https://doi.org/10.1080/1343943X.2015.1128095
- Tsegaw T; Hammes S; Robbertse J. Paclobutrazol-induced leaf, stem and root anatomical modification in potato. Hortscience 2005, 40, 1343–1346. https://doi.org/10.21273/HORTSCI.40.5.1343
- Tekalign T; Hammes P. Growth and biomass production in potato grown in the hot tropics as influenced by paclobutrazol. Plant Growth Regul. 2005, 45, 37–46. https://doi.org/10.1007/s10725-004-6443-1
- Fan Y, Chen J, Cheng Y, Raza MA, Wu X, Wang Z, et al. (2018). Effect of shading and light recovery on the growth, leaf structure, and photosynthetic performance of soybean in a maize-soybean relay-strip intercropping system. PLoS ONE 13:e0198159. https://doi.org/10.1371/journal.pone.0198159
- Wu Y, Gong W, Yang W (2017). Shade inhibits leaf size by controlling cell proliferation and enlargement in soybean. Sci. Rep. 7:9259. https://doi.org/10.1038/s41598-017-10026-5
- Kalve S, Fotschki J, Beeckman T, Vissenberg K, Beemster GT (2014). Three-dimensional patterns of cell division and expansion throughout the development of Arabidopsis thaliana leaves. J. Exp. Bot. 65, 6385–6397. https://doi.org/10.1093/jxb/eru358
- Terashima I, Hanba YT, Tazoe Y, Vyas,P, Yano S (2006). Irradiance and phenotype: comparative eco-development of sun and shade leaves in relation to photosynthetic CO2 diffusion. J. Exp. Bot. 57, 343–354 . https://doi.org/10.1093/jxb/erj014
- Qin YZ, Xing Z, Zou JF, He CZ, Li YL, Xiong XY (2014). Effects of sustained weak light on seedling growth and photosynthetic characteristics of potato seedlings. Scientia Agricultura Sinica. 47:537-545.
- Yao XY, Liu XY, Xu ZG, Jiao XL (2017). Effects of light intensity on leaf microstructure and growth of rape seedlings cultivated under a combination of red and blue LEDs. Journal of Integrative Agriculture. 16:97-105. https://doi.org/10.1016/S2095-3119(16)61393-X
- Taiz L, Zeiger E (2003). Plant physiology. 3rd edn. Annals of Botany. 91:750-751 https://doi.org/10.1093/aob/mcg079
- Pereira FJ, Castro EM, Oliveira C, Pires MF, Pasqual M, 2011. Mecanismos anatômicos e fisiológicos de plantas de aguapé para a tolerância à contaminação por Arsênio. Planta Daninha, 29;2:259-267.
- Tari, (2003) . Abaxidal and Adaxial stomatal density, stomatal conductances and water status of Bean primary leaves as Affected by paclobutrazol, Bio, plantarum. 47;215-220. https://doi.org/10.1023/B:BIOP.0000022254.63487.16
- Berova M, Zlatev Z. Physiological response and yield of paclobutrazol treated tomato plants (Lycopersicon esculentum Mill.). Plant Growth Regul. 2000, 30, 117–123. https://doi.org/10.1023/A:1006300326975
- Yadav, A., Solanki, D., Sharma, G., Dubey, Dr. G., & Sankhla, Dr. I. S. S. (2022). Phenotypic and Biochemical Characterization of Rhizobia Associated with Medicagopolymorpha Growing in Rajasthan. In Indian Journal of Advanced Botany (Vol. 2, Issue 2, pp. 5–11). https://doi.org/10.54105/ijab.b2012.102222
- Saranya, J., & Thenmozhi, Dr. N. (2020). Real Time CHIS Model for Efficient Sugarcane Plant Growth and Yield Estimation Model using Satellite Images. In International Journal of Recent Technology and Engineering (IJRTE) (Vol. 8, Issue 6, pp. 3101–3108). https://doi.org/10.35940/ijrte.f8454.038620
- Kogilavani, S. V., & Malliga, S. (2019). Deep Learning based Model for Plant Disease Detection. In International Journal of Innovative Technology and Exploring Engineering (Vol. 8, Issue 12, pp. 3416–3420). https://doi.org/10.35940/ijitee.l2585.1081219