Published January 1, 2023
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CD36 regulates substrates utilisation in brown adipose tissue of spontaneously hypertensive rats: in vitro study
Creators
- 1. Institute of Physiology, Czech Academy of Sciences,Prague, Czech Republic,
- 2. Institute of Physiology, Czech Academy of Sciences,Prague, Czech Republic
- 3. Institut klinicke a experimentalni mediciny
- 4. Department of Physiology, Faculty of Science, Charles University, Prague, Czech Republic
- 5. 1st Faculty of Medicine, Institute of Pathological Physiology, Charles University, Prague, Czech Republic
- 6. Azienda Ospedaliera Sant'Andrea
Description
Thermogenesis in brown adipose tissue (BAT) uses intracellular triglycerides, circulating free fatty acids and glucose as the main substrates. The objective of the current study was to analyse the role of CD36 fatty acid translocase in regulation of glucose and fatty acid utilisation in BAT. BAT isolated from spontaneously hypertensive rat (SHR) with mutant Cd36 gene and SHR-Cd36 transgenic rats with wild type variant was incubated in media containing labeled glucose and palmitate to measure substrate incorporation and oxidation. SHR-Cd36 versus SHR rats showed significantly increased glucose incorporation into intracellular lipids associated with reduced glycogen synthase kinase 3 beta (GSK-3 beta) protein expression and phosphorylation and increased oxidation of exogenous palmitate. It can be concluded that CD36 enhances glucose transport for lipogenesis in BAT by suppressing GSK-3 beta and promotes direct palmitate oxidation.
Notes
This work was supported by the Ministry of Health of the Czech Republic under its
Programme for the Conceptual Development of Research Organisations (Institute for Clinical and Experimental Medicine– IKEM, IN 00023001) to HMandbytheproject National Institute for Research of Metabolic and Cardiovascular
Diseases (Programme EXCELES, ID Project No. LX22NPO5104)- Funded by the European Union Next Generation EU to HM and MP. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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- Has metadata
- 37053180 (PMID)
- Is part of
- 1932-6203 (ISSN)
- 1932-6203 (ISSN)
- References
- 10.1371/journal.pone.0283276 (DOI)