Video records of experiments for "Layered DNA Data Storage"

These videos recorded our experiments on layered DNA data storage. The first color film “Becky Sharp” (53.59 MB) was encoded into ~10.08 million DNA strands. Simultaneous sequencing and real-time readout were achieved.

Supplementary Video 2: The full experiment process of real-time readout of the layered DNA storage with a panoramic view. This video demonstrates a complete real-time readout experiment, from the start of sequencing until the end of the film showing. It mainly covers three parts: signal acquisition, computing (base-calling and decoding), and film showing. The audio has been redacted.

Supplementary Video 3: The full experiment process of real-time readout of the layered DNA storage with a close view. This video illustrates a complete real-time film readout experiment from a close view, which mainly showcases sequencer signal acquisition and film showing. This same experiment process in Video 2 was recorded in Video 3 with another recorder. The audio has been redacted.

Supplementary Video 4: Film showing and statistics information monitoring (with audio in the recovered film). This video showcases the details of the film showing, consisting of playing and monitoring windows. The audio is from the restored film. After approximately 28.2 minutes (film readout latency, reading adapters and indices, refer to statistics information panel), the film began to stream out from DNA and playback. The film file was read out within approximately 16.7 minutes and it can be shown for approximately 30 minutes.

Supplementary Video 5: Signal acquisition and data transfer (with audio in the recovered film). This video presents the real-time signal acquisition of the sequencer and the data transfer of the sequencer server. The audio is from the restored film.

Supplementary Video 6: Flow-wise majority voting, layer-wise decoding, and streaming recovery (with audio in the recovered film). This video showcases the implementation details of flow-wise majority voting, layer-wise decoding, and streaming recovery in a real-time readout experiment. Meanwhile, the error rates of the recovered base coding layer were presented.

Supplementary Video 7: Signal analysis and base calling (16 nodes). This video illustrates the live signal analysis and incremental base calling. A total of 16 computing nodes equipped with 1,640 CPU cores and 24 NVIDIA A100 GPUs are deployed to balance the workload.

Supplementary Video 8: Detailed signal analysis and base calling (one node). To facilitate clearer illustration, Video 8 was zoomed in to obtain this Video. One of the 16 computing nodes was selected and illustrated.

Supplementary Video 9: Automatic template preparation on Ion Chef. This video showcases the use of the Ion Chef System for automated template preparation. After a total of 25 μL of a 20 pM oligo library was loaded, the instrument was started for automatic operation. Since the entire process was automated, this video only shows the first 2.5 hours.