Aqueous Two Phase Purification of Vigna radiata Amylase and its Characterization
Authors/Creators
- 1. Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida (UP), India.
Description
Amylase is digested the starch from the non-reducing end and thus producing limit dextrins
and maltose too which has industrial applications such as in food, pharmaceutical, paper,
leather, detergent and textile industries. Amylase was extracted and purified from Vigna
radiata (moong seeds) by a streamline method without the use of proteolytic and lipolytic
enzymes. The study features of present method were partial purification of crude enzyme
extract done by ammonium sulphate precipitation at low temperature followed by aqueous
two phase extraction by potassium phosphate-bi-phospahte buffer system with polyethylene
glycol to remove impurities. Enzyme activity of the extracted & purified Vigna radiata
amylase was done by dinitrosalicylic acid and absorbance was taken at 570 nm.
Abstract (English)
Amylase is digested the starch from the non-reducing end and thus producing limit dextrins
and maltose too which has industrial applications such as in food, pharmaceutical, paper,
leather, detergent and textile industries. Amylase was extracted and purified from Vigna
radiata (moong seeds) by a streamline method without the use of proteolytic and lipolytic
enzymes. The study features of present method were partial purification of crude enzyme
extract done by ammonium sulphate precipitation at low temperature followed by aqueous
two phase extraction by potassium phosphate-bi-phospahte buffer system with polyethylene
glycol to remove impurities. Enzyme activity of the extracted & purified Vigna radiata
amylase was done by dinitrosalicylic acid and absorbance was taken at 570 nm.
Files
IJCPR,Vol3,Issue3,Article2.pdf
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Additional details
Dates
- Accepted
-
2012-10-01