Hepato- and Nephroprotective Effects of the Polyphenol Concentrate From Cabernet Sauvignon Grape Varieties Cultivated in Kazakhstan in the Experimental Model Of CCL4-Induced Toxicity
Authors/Creators
- 1. PI "National Laboratory of Astana", Nazarbayev University, Astana, Kazakhstan, 010000
- 2. Nazarbayev University School of Medicine, Astana, Kazakhstan, 010000
- 3. National Center for Biotechnology, Astana, Kazakhstan, 010000
- 4. South Kazakhstan state pharmaceutical academy, Shymkent, Kazakhstan
Description
Objective: This study investigates the hepatoprotective effect and the antioxidant role of polyphenol concentrate in the experimental model of carbon tetrachloride (CCl4) induced toxicity. Methods: Antioxidant activity of Cabernet Sauvignon grape polyphenol were evaluated by radical scavenging of 1,1-diphenyl-2-picryl hydrazyl radical (DPPH), 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS.+). In addition, the effects of polyphenol concentrate on the survival of Wistar rats in the toxicity model, was also investigated. The polyphenol concentrate was administered for 5 five days prior to injection of carbon tetrachloride in a sub-lethal dose of 300 mg/kg of animal body weight in order to perform histological examinations of the liver and kidney, and detect the levels of AST, ALT and bilirubin. Results: Administration of polyphenol concentrate increased animal survival in the experimental model. Moreover, the intragastric administration of polyphenol concentrate prior to the initiation of the experimental model of toxicity, which was caused by a sub-lethal CCl4 dose, reduced morphological injuries in the liver and kidney, decreased the AST and ALT levels of the blood serum. Discussion and conclusion: Our data demonstrate that polyphenol concentrate possesses an antioxidant potential both in vitro and in vivo by reducing antioxidant stress that was caused by CCl4 administration into rats.
Abstract (English)
Objective: This study investigates the hepatoprotective effect and the antioxidant role of polyphenol concentrate in the experimental model of carbon tetrachloride (CCl4) induced toxicity. Methods: Antioxidant activity of Cabernet Sauvignon grape polyphenol were evaluated by radical scavenging of 1,1-diphenyl-2-picryl hydrazyl radical (DPPH), 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS.+). In addition, the effects of polyphenol concentrate on the survival of Wistar rats in the toxicity model, was also investigated. The polyphenol concentrate was administered for 5 five days prior to injection of carbon tetrachloride in a sub-lethal dose of 300 mg/kg of animal body weight in order to perform histological examinations of the liver and kidney, and detect the levels of AST, ALT and bilirubin. Results: Administration of polyphenol concentrate increased animal survival in the experimental model. Moreover, the intragastric administration of polyphenol concentrate prior to the initiation of the experimental model of toxicity, which was caused by a sub-lethal CCl4 dose, reduced morphological injuries in the liver and kidney, decreased the AST and ALT levels of the blood serum. Discussion and conclusion: Our data demonstrate that polyphenol concentrate possesses an antioxidant potential both in vitro and in vivo by reducing antioxidant stress that was caused by CCl4 administration into rats.
Files
IJTPR,Vol9,Issue3,Article1.pdf
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Additional details
Dates
- Accepted
-
2017-07-25
Software
References
- 1. Ebrahim S et al. eds. Epidemiology in Old Age, London: BMJ Publ. Group, 1996: 436 2. Rattan S.I. Aging is not a disease: implications for intervention. Aging Dis 2014; 5(Suppl.3): 196–202. 3. World Health Organization. Life in the 21st century: A vision for all (Report of the Director-General) 1998; 1- 14. 4. Moskalev A et al. Developing criteria for evaluation of geroprotectors as a key stage toward translation to the clinic. Aging Cell 2016; 15(Suppl.3): 407-415. 5. Moskalev AA, Pasyukova EG. From theories of aging to anti-aging interventions. Front Genet 2014; 5: 276. 6. Zs-Nagy I, Harman D, Kitani K. Pharmacology of Aging Process: Methods of Assessment and Potential Interventions, 1st ed. Ann. N.Y. Acad. Sci., 1994 7. Arutiunian AV, Kozina L.S. Mechanisms of free radical oxidation and its role in aging. Adv Gerontol 2009; 22(Suppl.1): 104–116. 8. Harman D. Free-radical theory of aging: increasing the functional life span. Ann NY Acad Sci 1994; 717: 1–15. 9. Wang CH et al. Oxidative stress response elicited by mitochondrial dysfunction: implication in the pathophysiology of aging. Exp Biol Med (Maywood) 2013; 238(Suppl.5): 450–460. 10.Panda SS et al. Xanthones as potential antioxidants. Curr Med Chem 2013; 20 (Suppl.36): 4481–4507. 11.Jayasena T et al. The role of polyphenols in the modulation of sirtuins. Ageing Res Rev 2013; 12(Suppl.4): 867–883. 12.Kasiotis KM et al. Resveratrol and related stilbenes: their anti-aging and anti-angiogenic properties. Food Chem Toxicol 2013; 61:112–120. 13.Marchal J et al. Resveratrol in mammals: effects on aging biomarkers, age-related diseases, and life span. Ann N Y Acad Sci 2013; 1290: 67–73. 14.Fraczek M et al. Resveratrol--phytophenol with wide activity. Pol Merkur Lekarski 2012; 32 (Suppl.188): 143-146. 15.Lin JK, Weng MS. Flavonoids as Nutraceuticals. In: Grotewold E., eds. The Science of Favonoids. New York: Springer Science+Business Media, Inc, 2006: 213–238. 16.Bhullar KS, Rupasinghe HP. Antioxidant and cytoprotective properties of partridgeberry polyphenols. Food Chem 2015; 168: 595-605. 17.Dani C et al. Intake of purple grape juice as a hepatoprotective agent in Wistar rats. J Med Food 2008; 11(Suppl.1): 12-32. 18.Kim Y et al. Antioxidant and cytoprotective effects of oligomeric and polymeric procyanidin fractions from defatted grape seed in PC12 cells. J Med Food 2012; 15(Suppl.5): 490-494. 19.Singha I, Das SK. Antioxidant potential of different grape cultivars against Fenton-like reagent-induced liver damage ex-vivo. Indian J BiochemBiophys 2014; 51(Suppl.5): 372-7. 20.Ky I et al. Wine by-products: phenolic characterization and antioxidant activity evaluation of grapes and grape pomaces from six different French grape varieties. Molecules 2014; 19(Suppl.1): 482–506. 21.Stockham K et al. Comparative studies on the antioxidant properties and polyphenolic content of wine from different growing regions and vintages, a pilot study to investigate chemical markers for climate change. Food Chem 2013; 40(Suppl.3): 500-506. 22.Weber LW et al. Hepatotixicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model. Crit Rev Toxicol 2013; 33(Suppl.2): 105-136 23.Hikita H et al. Activation of the Mitochondrial Apoptotic Pathway Produces Reactive Oxygen Species and Oxidative Damage in Hepatocytes That Contribute to Liver Tumorigenesis. Cancer Prev Res (Phila) 2015; 8(Suppl.8): 693-701. 24.Georgiev V et al. Recent Advances and Uses of Grape Flavonoids as Nutraceuticals. Nutrients 2014; 6(Suppl.1); 391–415. 25.Choudhary AK, Devi RS. Serum biochemical responses under oxidative stress of aspartame in Wistar albino rats Asian. Pac J Trop Dis 2014; 4 (Supp.1): 403–410 26.Hassan HM et al. The role of inflammatory and oxidative stress, CYP2E1 and bile acid disturbance in rat liver injury induced by isoniazid and lipopolysaccharide co-treatment. Antimicrob Agents Chemother 2016; 61 (Suppl.4). 27.Rajendran P et al. The Vascular Endothelium and Human Diseases. International Journal of Biological Sciences 2013; 9(Suppl.10): 1057–1069. 28.Sandur SK et al. Role of prooxidants and antioxidants in the anti-inflammatory and apoptotic effects of curcumin (diferuloylmethane). Free Radic Biol Med 2007; 43: 568–580. 29.Çetin A et al. Protective effect of hesperidin on oxidative and histological liver damage following carbon tetrachloride administration in Wistar rats. Arch Med Sci 2016; 12(Suppl.3): 486-493. 30.Sajid M et al. Proficiencies of Artemisia scoparia against CCl4 induced DNA damages and renal toxicity in rat. BMC Complement Altern Med. 2016; 16(Suppl.1): 149. 31.Recknagel RO et al. Mechanisms of carbon tetrachloride toxicity. Pharmacol Ther 1989; 43(Suppl.1): 139-154. 32.Deng JS et al. Hepatoprotective effect of the ethanol extract of Vitis thunbergii on carbon tetrachlorideinduced acute hepatotoxicity in rats through antioxidative activities. J Ethnopharmacol 2012; 142(Suppl.3): 795-803 33.Dogan A, Celik I. Hepatoprotective and antioxidant activities of grape seeds against ethanol-induced oxidative stress in rats. Br J Nutr 2012; 107(Suppl.1): 45-51. 34.Bak MJ et al. Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats. Int J Mol Sci 2016; 17(Suppl.5). 35.Madi AA, Farouk EM. Lipid-lowering and hepatoprotective effects of Vitis vinifera dried seeds on paracetamol-induced hepatotoxicity in rats. Nutr Res Pract 2015; 9(Suppl.1): 37-42. 36.Matsura T et al. Mechanisms of protection by melatonin against acetaminophen-induced liver injury in mice. J Pineal Res 2006; 41(Suppl.3): 211-219. 37.Charradi K et al. Protective effect of grape seed and skin extract against high-fat diet-induced liver steatosis and zinc depletion in rat. Dig Dis Sci 2014; 59(Suppl.8): 1768-1778. 38.Ismail AF et al. Hepatoprotective effect of grape seed oil against carbon tetrachloride induced oxidative stress in liver of γ-irradiated rat. J Photochem Photobiol B 2016; 160: 1-10.