Published December 30, 2017
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Effects of Tartrazine Subchronic Ingestion on Brush Border Membrane Enzymes of Female Swiss Albino Mice Intestine
Authors/Creators
- 1. Laboratory of Physiology of Nutrition and Food Safety, Department of Biology, Ahmed Ben Bella University of Oran 1, El Menaouer 31000 Oran, Algeria
- 2. Laboratory of Physiology of Nutrition and Food Safety, Department of Biology, Ahmed Ben Bella University of Oran 1, El Menaouer 31000 Oran, Algeria.
Description
Background: Tartrazine is a food colour that possesses adverse health effect to human such as hyperactivity in children, allergy and asthma. However, its effect on intestinal enzymes activity is not been established. Materials and methods: Tartrazine was administered to female swiss albino mice in drinking water at doses of 0.45% and 1% for 13 weeks. Mucosal scrape samples of the jejunum were collected for disaccharidases and dipeptidases activity analyses. Results: Maltase, sucrase and lactase specific activities were lower particularly in groups treated with 1% Tartrazine. Similarly, total and specific activities of L-leucyl-L-alanine, L-alanyl-L-proline and L-glycyl-L-proline dipeptidase are decreased in both treated groups. Villus height and crypt depth were decreased also in the same groups. Conclusion: the subchronic ingestion of Tartrazine at 0.45% and 1% in mice modifies disaccharidases and dipeptidases activities as well as intestine histological structure, thus it affects intestine function.
Abstract (English)
Background: Tartrazine is a food colour that possesses adverse health effect to human such as hyperactivity in children, allergy and asthma. However, its effect on intestinal enzymes activity is not been established. Materials and methods: Tartrazine was administered to female swiss albino mice in drinking water at doses of 0.45% and 1% for 13 weeks. Mucosal scrape samples of the jejunum were collected for disaccharidases and dipeptidases activity analyses. Results: Maltase, sucrase and lactase specific activities were lower particularly in groups treated with 1% Tartrazine. Similarly, total and specific activities of L-leucyl-L-alanine, L-alanyl-L-proline and L-glycyl-L-proline dipeptidase are decreased in both treated groups. Villus height and crypt depth were decreased also in the same groups. Conclusion: the subchronic ingestion of Tartrazine at 0.45% and 1% in mice modifies disaccharidases and dipeptidases activities as well as intestine histological structure, thus it affects intestine function.
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Dates
- Accepted
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2017-09-25
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References
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