Published September 30, 2018
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Journal article
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Alterations of Apoptotic and Epigenetic Genes Associated with Gatifloxacin-Induced Oxidative Stress in Rat Liver
Creators
- 1. Biochemistry Unit and Molecular Biology Research Laboratory, Department of Biological Sciences, Covenant University, Ota, Ogun State, Nigeria.
- 2. Biochemistry Unit and Molecular Biology Research Laboratory, Department of Biological Sciences, Covenant University, Ota, Ogun State, Nigeria
Description
In order to investigate the alterations in the expression of genes involved in epigenetics and apoptosis associated with gatifloxacin-induced oxidative stress in rat liver, adult rats were exposed to 10 mg/kg, 20 mg/kg, 40 mg/kg and 80 mg/kg gatifloxacin for five days orally. Biomarkers of oxidative stress were assessed spectrophotometrically while the levels of expression of Bcl2l1, caspases 3, 8 and 9 as well as Dnmt1, Hdac5, Prdm2, Eid3, Suv39h1 and Ehmt2 were assessed using relative reverse transcription polymerase chain reaction. The results showed that the dose-dependent increase in oxidative stress was associated with increase in the expression of proapoptotic genes. Gatifloxacin treatment also resulted in significant (p < 0.05) increase in the expression level of DNA and histone methylating genes. These changes observed at the lowest dosage of 10 mg/kg showed that gatifloxacin exposure could result in apoptosis and trigger epigenetic changes in the liver.
Abstract (English)
In order to investigate the alterations in the expression of genes involved in epigenetics and apoptosis associated with gatifloxacin-induced oxidative stress in rat liver, adult rats were exposed to 10 mg/kg, 20 mg/kg, 40 mg/kg and 80 mg/kg gatifloxacin for five days orally. Biomarkers of oxidative stress were assessed spectrophotometrically while the levels of expression of Bcl2l1, caspases 3, 8 and 9 as well as Dnmt1, Hdac5, Prdm2, Eid3, Suv39h1 and Ehmt2 were assessed using relative reverse transcription polymerase chain reaction. The results showed that the dose-dependent increase in oxidative stress was associated with increase in the expression of proapoptotic genes. Gatifloxacin treatment also resulted in significant (p < 0.05) increase in the expression level of DNA and histone methylating genes. These changes observed at the lowest dosage of 10 mg/kg showed that gatifloxacin exposure could result in apoptosis and trigger epigenetic changes in the liver.
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Additional details
Dates
- Accepted
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2018-02-23
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References
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