To Evaluate the Sensitivity, Specificity of Real Time PCR (TrueNAT) Assay in Case Detection of Mycobacterium Tuberculosis in Presumptive Pulmonary Tuberculosis Cases
Authors/Creators
- 1. Senior Resident, Jaipur National University Hospital IMSRC, Jaipur, Rajasthan
- 2. Assistant Professor, Jawaharlal Nehru Medical College, Ajmer, Rajasthan
- 3. Resident Doctor, Jawaharlal Nehru Medical College, Ajmer, Rajasthan
- 4. Senior Professor, Jawaharlal Nehru Medical College, Ajmer, Rajasthan
Description
Aim of Study: To evaluate the Sensitivity, Specificity of Real-time PCR (TrueNAT) assay in case detection of Mycobacterium Tuberculosis in Presumptive Pulmonary Tuberculosis Cases(Culture Positive). Material And Methods: This was a prospective, hospital based cross sectional study carried out in SCB Medical College and Hospital, Cuttack, Odisha for a period of one year, i.e from Sept 2019 –August 2020, on 50 Presumptive Pulmonary tuberculosis patients visiting inpatient and out-patient department of Pulmonary Medicine. Statical analysis of the data was conducted with Statistical Package for the Social Sciences (SPSS) VERSION 20.0. Results: Considering culture as the gold standard test the results of TrueNAT (RT PCR ) was compared for Pulmonary tuberculosis specimens in present study. Performance of Real-time PCR (TrueNAT ) assessed against Culture in this study, Sensitivity was 92.11%, Specificity was 91.67%. Conclusion: Our study concludes that Real Time PCR (TrueNAT) has shown good results and has high sensitivity and specificity for detection of Mycobacterium tuberculosis in Presumptive Pulmonary tuberculosis patients.
Abstract (English)
Aim of Study: To evaluate the Sensitivity, Specificity of Real-time PCR (TrueNAT) assay in case detection of Mycobacterium Tuberculosis in Presumptive Pulmonary Tuberculosis Cases(Culture Positive). Material And Methods: This was a prospective, hospital based cross sectional study carried out in SCB Medical College and Hospital, Cuttack, Odisha for a period of one year, i.e from Sept 2019 –August 2020, on 50 Presumptive Pulmonary tuberculosis patients visiting inpatient and out-patient department of Pulmonary Medicine. Statical analysis of the data was conducted with Statistical Package for the Social Sciences (SPSS) VERSION 20.0. Results: Considering culture as the gold standard test the results of TrueNAT (RT PCR ) was compared for Pulmonary tuberculosis specimens in present study. Performance of Real-time PCR (TrueNAT ) assessed against Culture in this study, Sensitivity was 92.11%, Specificity was 91.67%. Conclusion: Our study concludes that Real Time PCR (TrueNAT) has shown good results and has high sensitivity and specificity for detection of Mycobacterium tuberculosis in Presumptive Pulmonary tuberculosis patients.
Files
IJPCR,Vol15,Issue5,Article51.pdf
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Additional details
Dates
- Accepted
-
2023-04-30
Software
- Repository URL
- https://impactfactor.org/PDF/IJPCR/15/IJPCR,Vol15,Issue5,Article51.pdf
- Development Status
- Active
References
- 1. World Health Organisation. World Health Report 2004: Changing history Geneva: WHO. 2004. 2. Nikam C, Jagannath M, Narayanan MM, Ramanabhiraman V, KaziM, et al, Rapid Diagnosis of mycobacterium tuberculosis with TrueNAT MTB: A Near care Approach, PLoS ONE. 2013; 8(1): e51121. 3. Agarwal Y, Chopra RK, Gupta DK, Sethi RS. The tuberculosis timeline: Of white plague, a birthday present, and vignettes of myriad hues. Astrocyte. 2017; 4:7-26. 4. SH Siddiqui, Sabin R Gerdes, MGIT Procedure manual for BACTECTM MGIT 960TM TB System Page 9. 5. M. Singh. Development of TB Diagnostics in India: A Health innovation system perspective. 6. V. Mangayarkarasi, Sneka P, Sujith R and Jayaprakash, Ergonomic Diagnostic Tool based on chip Mini RT-PCR for Diagnosis of Pulmonary and Extra pulmonary tuberculosis, J Pure Appl Microbiol., 2019;13(2):1185-1190. 7. Kim MJ, Nam YS, Cho SY, Park TS, Lee HJ. Comparison of the Xpert MTB/RIF Assay and Real-time PCR for the Detection of Mycobacterium tuberculosis. Ann Clin Lab Sci. 2015 Spring; 45(3):327-32. 8. Nikam, C., Kazi, M., Nair, C., Jaggannath, M., M, M., R, V., Shetty, A., & Rodrigues, C. (2014). Evaluation of the Indian TrueNAT micro-RT-PCR device with GeneXpert for case detection of pulmonary tuberculosis. International Journal of Mycobacteriology. 2014; 3(3): 205–210.