Figure 4 in Phlebotomus (Paraphlebotomus) chabaudi and Phlebotomus riouxi: closely related species or synonyms?

Figure 4. Phylogenetic tree inferred from Phlebotomus chabaudi and Ph. riouxi specimens using the data of elongation factor 1-a gene. Sequences of Ph. chabaudi published by Tabbabi et al. (2014) were added to the analyses. The phylogram results from bootstrapped data sets obtained using the PhyML 3.0 program [21] using the HKY85 [25] + I (proportion of invariant sites) model. The tree was visualized using the TreeDyn program, version 198.3 [7]. Percentages shown above the branches are the frequencies at which a given branch appeared in 500 bootstrap replications. Only bootstrap values higher than 50% on the early branches are shown. A sequence of Ph. sergenti (EF416841) was used as the outgroup. The sequences marked by * were published by Tabbabi et al. (2014); R = sequences found in specimens morphologically characterized as Ph. riouxi. RC = sequences found in specimens morphologically characterized as Ph. chabaudi or Ph. riouxi. Int = sequences found in specimens morphologically characterized as intermediate between Ph. riouxi and Ph. chabaudi. RCint = sequences found in specimens morphologically characterized as Ph. riouxi, Ph. chabaudi and intermediate specimens between the two species.