Figures 28-40 in Oogenesis in Phragmatopoma (Polychaeta: Sabellariidae): Evidence for morphological distinction among geographically remote populations

Figures 28-40. Ultrastructure in Phragmatopoma caudata of the SW Atlantic. Growth Phase. Figure 28 Oocyte during late vitellogenesis filled with yolk granules (Y) and lipid droplets (Li). Note the cytoplasmic patches where the annulate lamellae (Al) are located and the welldeveloped egg envelope (Eg) (810x). Figure 29 Ripe oocytes occupy the entire coelomic cavity toward the body wall (810x). MC = muscle; Ep = epidermis. Figure 30 General view of the annulate lamellae (Al) between yolk granules and lipid droplets (560X). Figures 31-33 The annulate lamellae are composed by fenestrated endomembranes (arrows) parallel to each other arranged (Figure 31, 2,900X. Figure 32, 10,500X. Figure 33, 3,100X). Figure 34 Mature yolk granules with an elliptical shape (Y) showing a medullar crystalline substructure (white arrow) and non-crystalline cortex with electron-lucent spheres (black arrow) (13500X). Figure 35 Crystallised medullar in a parallel arrangement (white arrow) and a cortical lucid sphere (black arrow) (43,000X). Figure 36 Yolk granules (Y) and lipid droplets (Li) in the cytoplasm, with the presence of glycogen granules (arrow) close to the droplets (13,500X). M = mitochondria. Figure 37 Details of yolk granules delimited by the membrane (arrow) next to a lipid droplet (Li) and glycogen (Gly) (61000X). Figure 38 Cortical cytoplasm filled with yolk granules (Y), lipid droplets (Li) and cortical granules (CG) below the plasma membrane. Notice the thick egg envelope (Eg) (2,050X). Figure 39 The perivitelline space (1) is narrow and the egg envelope (Eg) is composed of two layers (2 and 3) with different electron-densities. The winding microvilli (Mv) are completely immersed in the egg envelope. Only the apical surface of the microvilli is in contact with the coelomic cavity (arrow) (5,400X). CG = cortical granules. Figure 40 Microvilli apex showing the expansion bears extensive filamentous adornment with an electron-dense centre (31,000X).