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Published June 2, 2017 | Version 2
Dataset Open

Human alpha-aminoadipic semialdehyde synthase (AASS); A Target Enabling Package

Description

This work provides the early tools to develop substrate reduction inhibitors for a genetic childhood seizure disorder, with the hypothesis to target the enzyme (AASS) upstream of the defective gene (ALDH7A1) in the human lysine metabolic pathway. This TEP package includes recombinant human AASS purification protocols, structures of the AASS second domain in different states, in vitro assays to detect ligand binding (differential scanning fluorimetry) and enzyme activity (NADH formation) of human AASS, as well as initial chemical matters.

Notes

This deposition represents version 2 of the TEP datasheet. Changes include, but are not limited to: Addition of hyperlinks throughout the body of the datasheet; Addition of Useful Links to relevant databases; Addition of a Reference box where applicable; Other modifications necessary for page spacing and visualisation. Please note, all data and results within the document remain the same as provided in version 1. To see version 1: https://doi.org/10.5287/bodleian:9R6aYwPGq

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AASS_TEP_datasheet_v2.pdf

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Additional details

Related works

Funding

Wellcome Trust
A UK Hub to Catalyse Open Target Discovery. 106169