Published December 18, 2018 | Version 1
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Thermolysin dataset collected with the helical scheme implemented in HEBI in the ZOO system

  • 1. RIKEN/SPring-8 Center

Description

Thermolysin is used as a standard crystal for evaluation of the automated data collection system ZOO developed at SPring-8 BL32XU.

Both edges of a crystal with size of 30×30×220 µm3 were automatically defined using 2(width)×15(height) µm2 beam. 

After dose estimation with KUMA by using the crystal size, other X-ray parameters and data collection parameters, shutter-less helical data collection was applied along the 3D vector consisted by the defined both crystal edges.

The intended absorbed dose for data collection was set to 8 MGy.

The crystals belonged to space group P6122 with unit cell parameters a=b=93.0, c=129.5, alpha,beta=90 gamma=120.

Data was processed up to 1.46 Å resolution in the published result (Hirata et al. accepted) using KAMO. 

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