Thermolysin dataset collected with the helical scheme implemented in HEBI in the ZOO system

Thermolysin is used as a standard crystal for evaluation of the automated data collection system ZOO developed at SPring-8 BL32XU.

Both edges of a crystal with size of 30×30×220 µm³ were automatically defined using 2(width)×15(height) µm² beam. 

After dose estimation with KUMA by using the crystal size, other X-ray parameters and data collection parameters, shutter-less helical data collection was applied along the 3D vector consisted by the defined both crystal edges.

The intended absorbed dose for data collection was set to 8 MGy.

The crystals belonged to space group P6₁22 with unit cell parameters a=b=93.0, c=129.5, alpha,beta=90 gamma=120.

Data was processed up to 1.46 Å resolution in the published result (Hirata et al. accepted) using KAMO.