Published February 22, 2018 | Version v1
Dataset Open

USP5 Zf-UBD Differential Scanning Fluorimetry Assay Development

  • 1. University of Toronto, Structural Genomics Consortium

Description

Development of DSF assay for USP5 Zf-UBD to determine optimal protein screening conditions and significance of thermal shift of USP5 Zf-UBD in the presence of a ubiquitin RLRGG peptide  

Notes

Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.

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20180222_USP5 DSF assay development.pdf

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