Published August 16, 2023
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Figure 2 in Agricultural Research Service Weed Science Research: Past, Present, and Future
Authors/Creators
- Young, Stephen L.
- Anderson, James V.
- Baerson, Scott R.
- Bajsa-Hirschel, Joanna
- Blumenthal, Dana M.1
- Boyd, Chad S.2
- Boyette, Clyde D.
- Brennan, Eric B.
- Cantrell, Charles L.
- Chao, Wun S.
- Chee-Sanford, Joanne C.
- Clements, Charlie D.
- Dray, F. Allen3
- Duke, Stephen O.
- Eason, Kayla M.4
- Fletcher, Reginald S.
- Fulcher, Michael R.
- Gaskin, John F.
- Grewell, Brenda J.
- Hamerlynck, Erik P.
- Hoagland, Robert E.
- Horvath, David P.
- Law, Eugene P.
- Madsen, John D.
- Martin, Daniel E.
- Mattox, Clint
- Mirsky, Steven B.
- Molin, William T.
- Moran, Patrick J.
- Mueller, Rebecca C.
- Nandula, Vijay K.
- Newingham, Beth A.
- Pan, Zhiqiang
- Porensky, Lauren M.1
- Pratt, Paul D.
- Price, Andrew J.
- Rector, Brian G.
- Reddy, Krishna N.
- Sheley, Roger L.2
- Smith, Lincoln
- Smith, Melissa C.3
- Snyder, Keirith A.
- Tancos, Matthew A.
- West, Natalie M.
- Wheeler, Gregory S.
- Williams, Martin M.
- Wolf, Julie
- Wonkka, Carissa L.
- Wright, Alice A.
- Xi, Jing
- Ziska, Lew H.
- 1. 29 33 29 16
- 2. 29
- 3. 32 17 34 35 36 37 34 38
- 4. 16 17 18, 19 20 21 22 23 24, 25 26 27 28, 29 30 31 32
Description
Figure 2. (A) Depiction of a bipartite synthetic Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) cassette (red) consisting of a Cas9 endonuclease and a guide ribonucleic acid RNA (gRNA) that is flanked by homology arms (HA) (black). (B) Following expression of the CRISPR cassette, gRNA binds to Cas9 and directs the complex to a unique sequence-specific site for DNA cleavage and homology-directed repair (HDR). Following HDR, the CRISPR cassette is copied into both genomic regions. (C) Standard Mendelian inheritance results in 50% of progeny inheriting a modified gene. In contrast, a gene drive would bias inheritance, theoretically resulting in all progenies (~99%) inheriting the modified gene, thereby "driving" the modified gene into an invasive weed population.
Notes
Published as part of Young, Stephen L., Anderson, James V., Baerson, Scott R., Bajsa-Hirschel, Joanna, Blumenthal, Dana M., Boyd, Chad S., Boyette, Clyde D., Brennan, Eric B., Cantrell, Charles L., Chao, Wun S., Chee-Sanford, Joanne C., Clements, Charlie D., Dray, F. Allen, Duke, Stephen O., Eason, Kayla M., Fletcher, Reginald S., Fulcher, Michael R., Gaskin, John F., Grewell, Brenda J., Hamerlynck, Erik P., Hoagland, Robert E., Horvath, David P., Law, Eugene P., Madsen, John D., Martin, Daniel E., Mattox, Clint, Mirsky, Steven B., Molin, William T., Moran, Patrick J., Mueller, Rebecca C., Nandula, Vijay K., Newingham, Beth A., Pan, Zhiqiang, Porensky, Lauren M., Pratt, Paul D., Price, Andrew J., Rector, Brian G., Reddy, Krishna N., Sheley, Roger L., Smith, Lincoln, Smith, Melissa C., Snyder, Keirith A., Tancos, Matthew A., West, Natalie M., Wheeler, Gregory S., Williams, Martin M., Wolf, Julie, Wonkka, Carissa L., Wright, Alice A., Xi, Jing & Ziska, Lew H., 2023, Agricultural Research Service Weed Science Research: Past, Present, and Future, pp. 312-327 in Weed Science (Cambridge, England) 71 (4) on page 318, DOI: 10.1017/wsc.2023.31, http://zenodo.org/record/11710141
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- Is part of
- Journal article: 10.1017/wsc.2023.31 (DOI)
- Journal article: urn:lsid:plazi.org:pub:FFF7FF9BFF9BCB09FFAFFFFEF234433A (LSID)
- Journal article: https://zenodo.org/record/11710141 (URL)