To Study the Oxidative Stress & It's Correlation with Vit B12 & Folic Acid Levels in Megaloblastic Anemia
Creators
- 1. PG Resident 1st Year, Department of Biochemistry, Govt. Medical College, Datia, Madhya Pradesh, India
- 2. Professor, Department of Biochemistry, Govt. Medical College. Datia, Madhya Pradesh, India
- 3. Demonstrator, Department of Biochemistry, MLB Medical College Jhansi, U.P, India
- 4. Assistant Professor, Department of Microbiology, Govt. Medical College Datia, Madhya Pradesh, India
Description
Introduction: WHO defines, megaloblastic anemia as a type of anemia characterized by the formation of unusually large, abnormal and immature red blood cells. Prevalence of megaloblastic anemia in India according to many studies ranges from 02%-40%.It has been found to have decrease antioxidant activity in anemic patient causing increase oxidative stress, leading to DNA damage and altered biomolecules. Aim: To study the oxidative stress & it’s correlation with vitamin B12 & folic acid levels in Megaloblastic anemia. Methods: A Case Control Study was carried out from January 2023 to June 2023 by Medicine & Biochemistry department of Government Medical College, Datia(Madhya Pradesh) .100 non-pregnant female(age ranges 15-45yrs) patient having Hb<12g/dl & Megaloblastic anemia were taken as cases. Whereas 100, age and sex matched without anemia as controls. Blood sample was collected in EDTA vial for complete blood count & peripheral blood smear. Blood sample was collected in plain vial to estimate serum vitamin B12, folate & FRAP (Ferric Reducing Ability of Plasma). Serum B12 & folate estimation was done by Chemiluminiscence method & Spectrophotometric analysis was done for FRAP. Results: Serum Vitamin B12 was found to be lower among 56% of cases (113.2±189.6) compared to controls (206.7±261.9). Serum Folate was found to be lower among 25% of cases (5.7±4.1) compared to controls (7.3±4.2).Both vitamin B 12 & folic acid, found to low among 19% of cases. Serum FRAP value was found to be lower among Cases (582.7±198) compared to controls (908.1±174.2).There was a positive significant (p-value<0.05) & mild correlation of Serum FRAP value with serum Vitamin B12 and Serum Folate in Controls. In Cases the correlation was stronger with positive significance (p-value<0.05), compared to Controls. Conclusion: In our study, it may be concluded that in Megaloblastic anaemia there is serum folate and Vitamin B12 deficiency with increased oxidative stress leading to free radical generation and peroxidation of vital body molecules. Therefore in such cases, thorough biochemical analysis should be undertaken before starting with medications and there should be an antioxidant supplementation to be given.
Abstract (English)
Introduction: WHO defines, megaloblastic anemia as a type of anemia characterized by the formation of unusually large, abnormal and immature red blood cells. Prevalence of megaloblastic anemia in India according to many studies ranges from 02%-40%.It has been found to have decrease antioxidant activity in anemic patient causing increase oxidative stress, leading to DNA damage and altered biomolecules. Aim: To study the oxidative stress & it’s correlation with vitamin B12 & folic acid levels in Megaloblastic anemia. Methods: A Case Control Study was carried out from January 2023 to June 2023 by Medicine & Biochemistry department of Government Medical College, Datia(Madhya Pradesh) .100 non-pregnant female(age ranges 15-45yrs) patient having Hb<12g/dl & Megaloblastic anemia were taken as cases. Whereas 100, age and sex matched without anemia as controls. Blood sample was collected in EDTA vial for complete blood count & peripheral blood smear. Blood sample was collected in plain vial to estimate serum vitamin B12, folate & FRAP (Ferric Reducing Ability of Plasma). Serum B12 & folate estimation was done by Chemiluminiscence method & Spectrophotometric analysis was done for FRAP. Results: Serum Vitamin B12 was found to be lower among 56% of cases (113.2±189.6) compared to controls (206.7±261.9). Serum Folate was found to be lower among 25% of cases (5.7±4.1) compared to controls (7.3±4.2).Both vitamin B 12 & folic acid, found to low among 19% of cases. Serum FRAP value was found to be lower among Cases (582.7±198) compared to controls (908.1±174.2).There was a positive significant (p-value<0.05) & mild correlation of Serum FRAP value with serum Vitamin B12 and Serum Folate in Controls. In Cases the correlation was stronger with positive significance (p-value<0.05), compared to Controls. Conclusion: In our study, it may be concluded that in Megaloblastic anaemia there is serum folate and Vitamin B12 deficiency with increased oxidative stress leading to free radical generation and peroxidation of vital body molecules. Therefore in such cases, thorough biochemical analysis should be undertaken before starting with medications and there should be an antioxidant supplementation to be given.
Files
IJPCR,Vol15,Issue8,Article203.pdf
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Additional details
Dates
- Accepted
-
2023-08-20
Software
- Repository URL
- https://impactfactor.org/PDF/IJPCR/15/IJPCR,Vol15,Issue8,Article203.pdf
- Development Status
- Active
References
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