Published May 30, 2024
| Version v1
Dataset
Open
Zeiss Lattice Light Sheet 7 Point Spread Functions and example dataset
Creators
- 1. Bioimaging and Optics Core Facility, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
Description
This repository contains some point spread functions distilled from imaging fluorescent beads, 200nm, F8811 in an agarose gel, on a Zeiss Lattice Light sheet 7 microscope.
Several images were acquired with varying settings of the light sheet (light sheet was always set as the 30 um x1000 nm).
These three parameters were varied:
FS: focus sheet
FW: focus waist
AC: aberration control
The file psf-200nm correspond to the best settings taken between the different settings. The two other files (psf-300nm, psf-400nm) correspond to resampled versions of the psf-200nm file.
A sample czi file (Hela Kyoto, raw data) is provided to test deconvolution algorithms.
Note that none of these data is deskewed.
Files
30x1000_FS+000_FW60_AC170_psf.tif
Files
(4.6 GB)
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