Harnessing Nature's Defence: The Antimicrobial Efficacy of Pasteurised Cattle Milk-Derived Extracellular Vesicles on Staphylococcus aureus ATCC 25923
Creators
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1.
Estonian University of Life Sciences
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2.
University of Tartu
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3.
University of Peradeniya
- 4. Institute of Animal Reproduction and Food Research, Polish Academy of Sciences / Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk
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5.
University of Sheffield
Description
Increasing antimicrobial resistance (AMR) challenges conventional antibiotics, prompting the search for alternatives. Extracellular vesicles (EVs) from pasteurised cattle milk offer promise, due to their unique properties. This study investigates their efficacy against five pathogenic bacteria, including Staphylococcus aureus ATCC 25923, aiming to combat AMR and to develop new therapies. EVs were characterised and tested using various methods. Co-culture experiments with S. aureus showed significant growth inhibition, with colony-forming units decreasing from 2.4 × 105 CFU/mL (single dose) to 7.4 × 104 CFU/mL (triple doses) after 12 h. Milk EVs extended lag time (6 to 9 h) and increased generation time (2.8 to 4.8 h) dose-dependently, compared to controls. In conclusion, milk EVs exhibit dose-dependent inhibition against S. aureus, prolonging lag and generation times. Despite limitations, this suggests their potential in addressing AMR.
Files
ijms-25-04759-v2.pdf
Files
(3.6 MB)
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Additional details
Funding
- COMBIVET – Setting up the ERA Chair of Comparative Medicine in the Institute of Veterinary Medicine and Animal Sciences of the Estonian University of Life Sciences 857418
- European Commission
- OH-Boost – Boosting the One Health Research Excellence and Management Capacity of the Estonian University of Life Sciences 101079349
- European Commission
- Effects and mechanisms of plant bioactive substances in foods of animal origin PRG1441
- Estonian Research Council
Dates
- Submitted
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2024-04-04