Published December 30, 2023 | Version v1
Journal article Open

Isolation and Identification of Fungi from Patients Suffering from Superficial Skin Infection in a Tertiary Care Hospital

  • 1. 1 Subject Co-Ordinator, Department of Education, Ministry of Education, Madhya Pradesh
  • 2. 2 Professor & Head, Department of Microbiology, NAMO Medical Education and Research Institute, Silvassa, DNH
  • 3. Associate Professor, Department of Microbiology, NAMO Medical Education and Research Institute, Silvassa, DNH.
  • 4. Assistant Professor, Department of Microbiology, NAMO Medical Education and Research Institute, Silvassa, DNH.
  • 5. Tutor, Department of Microbiology, NAMO Medical Education and Research Institute, Silvassa, DNH

Description

Introduction: Superficial fungal infections are often seen in day-to-day clinical practice, and their prevalence
continues to rise worldwide. The present study attempts to find out the distribution of superficial fungal infection
with respect to socio-economic and demographic variables among a symptomatic group.
Objective: The present study was undertaken with a view to isolate the fungi from patients suffering from
superficial skin infection attending Dermatology OPD at NAMO Medical Education & Research Institute
Silvassa, DNH and to assess the clinical profile, prevalence of dermatophytes infection in study population.
Methods: A clinical and mycological study of superficial mycosis was conducted on 100 cases (65 male & 35
female). Direct microscopy by KOH mount and culture was undertaken to isolate the fungal pathogen in each
case. Further, LPCB mount was performed for microscopic morphology.
Result: A total of 100 patients were enrolled in the study. Dermatophytosis was the commonest superficial
fungal infection in 47 cases), followed by Candidiasis in 8 (14.5%) cases. Commonest clinical presentation was
T. corporis in 32 (39%), followed by T. cruris in 27 (33%) cases. The commonest dermatophyte cultured was T.
mentagrophyte in 22 (22%) followed by T. rubrum in 15 (14.9%) cases. Non Dermatophytic Molds (NDM) were
isolated in 3/55 (5.4%) cases. The commonest NDM isolated was Exophiala.
Conclusion: We conclude that along with dermatophytes, non dermatophytic fungus are also emerging as
important cause of superficial mycosis. Direct microscopy and culture both are important tools for diagnosis of
fungal infections.

Abstract (English)

Introduction: Superficial fungal infections are often seen in day-to-day clinical practice, and their prevalence
continues to rise worldwide. The present study attempts to find out the distribution of superficial fungal infection
with respect to socio-economic and demographic variables among a symptomatic group.
Objective: The present study was undertaken with a view to isolate the fungi from patients suffering from
superficial skin infection attending Dermatology OPD at NAMO Medical Education & Research Institute
Silvassa, DNH and to assess the clinical profile, prevalence of dermatophytes infection in study population.
Methods: A clinical and mycological study of superficial mycosis was conducted on 100 cases (65 male & 35
female). Direct microscopy by KOH mount and culture was undertaken to isolate the fungal pathogen in each
case. Further, LPCB mount was performed for microscopic morphology.
Result: A total of 100 patients were enrolled in the study. Dermatophytosis was the commonest superficial
fungal infection in 47 cases), followed by Candidiasis in 8 (14.5%) cases. Commonest clinical presentation was
T. corporis in 32 (39%), followed by T. cruris in 27 (33%) cases. The commonest dermatophyte cultured was T.
mentagrophyte in 22 (22%) followed by T. rubrum in 15 (14.9%) cases. Non Dermatophytic Molds (NDM) were
isolated in 3/55 (5.4%) cases. The commonest NDM isolated was Exophiala.
Conclusion: We conclude that along with dermatophytes, non dermatophytic fungus are also emerging as
important cause of superficial mycosis. Direct microscopy and culture both are important tools for diagnosis of
fungal infections.

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Dates

Accepted
2023-12-19