Published May 24, 2024 | Version v4
Dataset Open

DeAbePlusData

Creators

Description

Two sets of example data for DeAbe and multi-step models are provided.

Data #1: fixed cells (zipped file: "FixedCell_PtK2.rar")

  1. Fixed cells with actin labeled acquired with a lattice light sheet microscope with 1.0NA objective.
  2. Raw image voxel size: 0.108um x 0.108um x 0.215um.
  3. Data includes training images, test images, and benchmark images with aberration correct by AO. 

Data #2: C. elegans embryos (zipped files: "Step1_DL_DeAbe.rar",  "Step2_DL_Decon.rar", "Step3_DL_Iso.rar", and "Test_Data.rar")

  1. Data were from C elegans embryos expressing a membrane marker (strain od58).
  2. Images were acquired with dual-view light sheet microscope (diSPIM) with 0.8NA + 0.8NA objectives.
  3. Raw image voxel size: 0.1625um x 0.1625um x 1.0um.
  4. In the raw image of SPIMA, the first slice of the image stack corresponds to the physically deepest side of the embryo, and the last slice coresponds to the most shallow side of the embryos. In contrast, in the raw image of SPIMB, the last slice of the image stack corresponds to the physically deepest side of the embryo, and the first slice corresponds to the most shallow side of the embryos.
  5. This data is for the multi-step processing pipeline, including:

    1) Step1 DL DeAbe: DL based de-aberration, with 3D-RCAN network.

    2) Step2 DL Decon: DL based deconvoltuion to recover image quality of dual-view joint deconvolution, with 3D-RCAN network.

    3) Step3 DL Iso: DL based axial resolution enhancement to achieve isotropic resolution, with CARE network.

    4) Test data.

Files

README.txt

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