Evaluation of Cells in Cerebro Spinal Fluid for Postmortem Interval
Creators
- 1. Assistant Professor, Dept. of Forensic Medicine and Toxicology, Maharishi Vashishtha Autonomous State Medical College, Basti
- 2. Assistant Professor, Department of Pharmacology, Prasad Institute of Medical Sciences, Lucknow
- 3. Additional Professor, Emergency Medicine Department, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow
- 4. Assistant Professor, Dept. of Forensic Medicine and Toxicology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow
Description
Background: The post-mortem interval, often known as the time since death, is the period of time between when a person passes away and when the body is examined by a forensic expert. Objectives: To estimate the total count and differential count of cells in the cerebrospinal fluid with cytological differences. Methods: This prospective study was conducted in the Department of Forensic Medicine in a medical college for a period of 6 months. This study analyzed the CSF from the cerebellopontine cistern for cell count and morphology in all medicolegal autopsies with known time since death by Neubaur chamber and light microscopy. The study sample consisted of 40 CSF samples taken from medicolegal autopsies. Ethical clearance was obtained from the Institutional Ethical committee (IEC) before starting the study. All cases subjected for medico-legal autopsy whose time of death is known were included in the study. Results: Most common cause is natural causes comprising 36%, followed by poisoning in 28%. Hanging and trauma were other common causes. Out of 40 patients (27 were males and 13 were females) so the study was female preponderance. The most common age group was 21-40 years in 48.71% followed by 41-60 years in 28%. The mean value of total cell count in CSF in PMI of 0-6 hours is 250 with a standard error of 28.86. The mean value of total cell count in PMI 6 – 12 hours is 388.81 with a standard error of 37.36. The mean differential cell count in cases with PMI 0 – 6 hours shows that mean neutrophils count is 23.33, mean lymphocyte count is 70 and the mean eosinophil count id 6.66. There were no degenerated cells in this period The mean differential cell count in cases with PMI 6 – 12 hours shows that the mean neutrophil count is 12.72; the mean lymphocyte count is 75.45. The mean degenerated cells in this period are 11.81. Conclusion: According to the study, the post-mortem increase in CSF cell count is a typical physiological occurrence. Up to 12 hours after the injury, the CSF cell count increases, but the first 6 hours show no degenerative alterations hours. Neutrophils, lymphocytes, plasma cells, eosinophils, and histiocytes are the distinguishable cells.
Abstract (English)
Background: The post-mortem interval, often known as the time since death, is the period of time between when a person passes away and when the body is examined by a forensic expert. Objectives: To estimate the total count and differential count of cells in the cerebrospinal fluid with cytological differences. Methods: This prospective study was conducted in the Department of Forensic Medicine in a medical college for a period of 6 months. This study analyzed the CSF from the cerebellopontine cistern for cell count and morphology in all medicolegal autopsies with known time since death by Neubaur chamber and light microscopy. The study sample consisted of 40 CSF samples taken from medicolegal autopsies. Ethical clearance was obtained from the Institutional Ethical committee (IEC) before starting the study. All cases subjected for medico-legal autopsy whose time of death is known were included in the study. Results: Most common cause is natural causes comprising 36%, followed by poisoning in 28%. Hanging and trauma were other common causes. Out of 40 patients (27 were males and 13 were females) so the study was female preponderance. The most common age group was 21-40 years in 48.71% followed by 41-60 years in 28%. The mean value of total cell count in CSF in PMI of 0-6 hours is 250 with a standard error of 28.86. The mean value of total cell count in PMI 6 – 12 hours is 388.81 with a standard error of 37.36. The mean differential cell count in cases with PMI 0 – 6 hours shows that mean neutrophils count is 23.33, mean lymphocyte count is 70 and the mean eosinophil count id 6.66. There were no degenerated cells in this period The mean differential cell count in cases with PMI 6 – 12 hours shows that the mean neutrophil count is 12.72; the mean lymphocyte count is 75.45. The mean degenerated cells in this period are 11.81. Conclusion: According to the study, the post-mortem increase in CSF cell count is a typical physiological occurrence. Up to 12 hours after the injury, the CSF cell count increases, but the first 6 hours show no degenerative alterations hours. Neutrophils, lymphocytes, plasma cells, eosinophils, and histiocytes are the distinguishable cells.
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IJTPR,Vol13,Issue3,Article44.pdf
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Additional details
Dates
- Accepted
-
2023-03-14
Software
References
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