A Study on Seroprevalence of Dengue Virus in a Tertiary Care Hospital in North Karnataka
Creators
- 1. Assistant Professor, Department of Pathology, ESIC Medical College and Hospital Kalaburagi
- 2. Associate Professor, Department of General Medicine, ESIC Medical College and Hospital Kalaburagi
- 3. Associate Professor, Department of Pathology, ESIC Medical College and Hospital Kalaburagi
Description
Introduction: Dengue fever is an acute viral disease caused by Dengue virus of the Flaviviridae family and is transmitted by the bite of an infected female mosquito Aedes aegypti. The Dengue virus causes significant morbidity and mortality in many parts of the world, including India. Though, the cases of dengue fever are mild and self-resolving, there can be fatal complications like Dengue Haemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS). The aim of this study is to calculate the seroprevalence of Dengue fever referred to tertiary care hospital in North Karnataka. Materials and Methods: This is a prospective type of cross-sectional study conducted in Department of Medicine for a period of two years from June 2021 to May 2023 at ESIC Medical College And Hospital, Kalaburagi, North Karnataka, India. The serum samples were collected from suspected dengue fever cases and tested by Immunoglobulin M (IgM) capture Enzyme Linked Immunosorbent Assay (ELISA) to detect IgM antibody against dengue virus and NS1 for dengue NS1 (Non-Structural Protein-1) antigen using Standard ELISA kits. The statistical analysis was done with SPSS 26.0 version. Results: A total of 5940 serum samples were screened over a period of two years for Dengue IgM and IgG. Most affected age group was between 10-20 years and male to female ratio was 1.2:1. A total of 1040 (17.50%) cases were positive for dengue infection. Among which 812(80.3%) cases were positive for anti-dengue IgM antibodies, 96 (9.23%)cases were positive for NS1 antigen and 132 (12.70%)cases were positive for both, respectively. Conclusion: Regular epidemiological studies are necessary to monitor the dengue situation in high risk areas. Seroprevalence of dengue infection being critical signifies the importance of detection of both IgM antibodies and NS1 antigen for diagnosis of dengue infection to prevent fatal complications like Dengue Haemorrhagic Fever and Dengue Shock Syndrome . The study identifies younger population being at higher risk and most cases were reported during the during monsoon period, which warrants coordinated action toward vector control measures.
Abstract (English)
Introduction: Dengue fever is an acute viral disease caused by Dengue virus of the Flaviviridae family and is transmitted by the bite of an infected female mosquito Aedes aegypti. The Dengue virus causes significant morbidity and mortality in many parts of the world, including India. Though, the cases of dengue fever are mild and self-resolving, there can be fatal complications like Dengue Haemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS). The aim of this study is to calculate the seroprevalence of Dengue fever referred to tertiary care hospital in North Karnataka. Materials and Methods: This is a prospective type of cross-sectional study conducted in Department of Medicine for a period of two years from June 2021 to May 2023 at ESIC Medical College And Hospital, Kalaburagi, North Karnataka, India. The serum samples were collected from suspected dengue fever cases and tested by Immunoglobulin M (IgM) capture Enzyme Linked Immunosorbent Assay (ELISA) to detect IgM antibody against dengue virus and NS1 for dengue NS1 (Non-Structural Protein-1) antigen using Standard ELISA kits. The statistical analysis was done with SPSS 26.0 version. Results: A total of 5940 serum samples were screened over a period of two years for Dengue IgM and IgG. Most affected age group was between 10-20 years and male to female ratio was 1.2:1. A total of 1040 (17.50%) cases were positive for dengue infection. Among which 812(80.3%) cases were positive for anti-dengue IgM antibodies, 96 (9.23%)cases were positive for NS1 antigen and 132 (12.70%)cases were positive for both, respectively. Conclusion: Regular epidemiological studies are necessary to monitor the dengue situation in high risk areas. Seroprevalence of dengue infection being critical signifies the importance of detection of both IgM antibodies and NS1 antigen for diagnosis of dengue infection to prevent fatal complications like Dengue Haemorrhagic Fever and Dengue Shock Syndrome . The study identifies younger population being at higher risk and most cases were reported during the during monsoon period, which warrants coordinated action toward vector control measures.
Files
IJPCR,Vol15,Issue10,Article58.pdf
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Additional details
Dates
- Accepted
-
2023-10-05
Software
- Repository URL
- https://impactfactor.org/PDF/IJPCR/15/IJPCR,Vol15,Issue10,Article58.pdf
- Development Status
- Active
References
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