Study of Assessment of P16 Expression in Various Grades of Squamous Cell Carcinoma of Head and Neck
Authors/Creators
- 1. Assistant Professor, Department of Pathology, Government Dharmapuri Medical College, Dharmapuri
Description
Introduction: p16 is a tumor suppressor gene, over expression of which is considered as a surrogate marker of oncogenic human papillomavirus (HPV) infection. Moreover, p16 over expression correlates with good prognosis in head and neck squamous cell carcinoma (HNSCC). In the present study, we aimed to evaluate the frequency of p16 overexpression in HNSCC in our institute and its association with clinicopathologic parameters. Methods: We performed p16 immunohistochemistry (IHC) on 40 cases of HNSCC in a tertiary care teaching hospital. Association of p16 over expression with various clinicopathologic parameters was evaluated. Results: In our study the most common site involved was oral cavity in 11 patients followed by sino-nasal region in 7 patients. Ca Nasopharynx was diagnosed in 6 patients, Ca oropharynx in 5 patients and lip was involved in 4 patient. In our study p16 over expression was noted in 40% (14 cases), while 60% (26 cases) were negative for p16 over expression. The relationship between histopathological type of squamous cell carcinoma and p16 immunohistochemistry is statistically in significant. Conclusion: HPV16 is the subtype most every now and again connected with HNC, and current clinically pertinent recognition strategies center around distinguishing the presence of HPV16 by PCR or ISH, or identification of p16 protein articulation in tumor tests by IHC. Moreover, p16 expression was found to be associated with some good prognostic parameters like lack of nodal metastasis; however, no significant association was noted with overall disease-free survival.
Abstract (English)
Introduction: p16 is a tumor suppressor gene, over expression of which is considered as a surrogate marker of oncogenic human papillomavirus (HPV) infection. Moreover, p16 over expression correlates with good prognosis in head and neck squamous cell carcinoma (HNSCC). In the present study, we aimed to evaluate the frequency of p16 overexpression in HNSCC in our institute and its association with clinicopathologic parameters. Methods: We performed p16 immunohistochemistry (IHC) on 40 cases of HNSCC in a tertiary care teaching hospital. Association of p16 over expression with various clinicopathologic parameters was evaluated. Results: In our study the most common site involved was oral cavity in 11 patients followed by sino-nasal region in 7 patients. Ca Nasopharynx was diagnosed in 6 patients, Ca oropharynx in 5 patients and lip was involved in 4 patient. In our study p16 over expression was noted in 40% (14 cases), while 60% (26 cases) were negative for p16 over expression. The relationship between histopathological type of squamous cell carcinoma and p16 immunohistochemistry is statistically in significant. Conclusion: HPV16 is the subtype most every now and again connected with HNC, and current clinically pertinent recognition strategies center around distinguishing the presence of HPV16 by PCR or ISH, or identification of p16 protein articulation in tumor tests by IHC. Moreover, p16 expression was found to be associated with some good prognostic parameters like lack of nodal metastasis; however, no significant association was noted with overall disease-free survival.
Files
IJPCR,Vol15,Issue10,Article6.pdf
Files
(2.8 MB)
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Additional details
Dates
- Accepted
-
2023-09-30
Software
- Repository URL
- https://impactfactor.org/PDF/IJPCR/15/IJPCR,Vol15,Issue10,Article6.pdf
- Development Status
- Active
References
- 1. Global cancer statistics, 2002. Parkin DM, Bray F, Ferlay J, Pisani P. CA Cancer J Clin. 2005; 55:74–108. 2. Diagnostic accuracy of touch imprint cytology for head and neck malignancies: a useful intraoperative tool in resource limited countries. Naveed H, Abid M, Hashmi AA, Edhi MM, Sheikh AK, Mudassir G, Khan A. BMC Clin Pathol. 2017; 25:17. 3. Head and neck squamous cell carcinomacomparative evaluation of pathological parameters in young and old patients. Naz S, Salah K, Khurshid A, Hashmi AA, Faridi N. Asian Pac J Cancer Prev. 2015;16:4061–4063. 4. Distinct risk factor profiles for human papillomavirus type 16-positive and human papillomavirus type 16-negative head and neck cancers. Gillison ML, D'Souza G, Westra W, Sugar E, Xiao W, Begum S, Viscidi R. J Nat Can Inst. 2008;100:407–420. 5. 5.Bahl A, Kumar P, Dar L, Mohanti BK, Sharma A, Thakar A, et al. Prevalence and trends of human papillomavirus in oropharyngeal cancer in a predominantly north Indian population. Head Neck. 2014; 36(4): 505-10. 6. Datta J., Smith A., Lang J.C., Islam M., Dutt D., Teknos T.N., Pan Q. microRNA-107 functions as a candidate tumor-suppressor gene in head and neck squamous cell carcinoma by downregulation of protein kinase Cɛ Oncogene. 2012;31:4045–4053 7. Boslooper K, Lam AK, Gao J, Weinstein S, Johnson N. The clinicopathological roles of alpha-B-crystallin and p53 expression in patients with head and neck squamous cell carcinoma. Pathology. 2008; 40:500–4. 8. 20 Wilson GD, Saunders MI, Dische S, Bentzen SM, Richman PI, Daley FM. Bcl2 expression in head and neck cancer. Int J Radiat Oncol Biol Phys. 2001; 49:435–41. 9. Thambiah LJ, Bindushree RV, Anjum A, Pugazhendi SK, Babu L, Nair RP. Evaluating the expression of p16 and p27 in oral epithelial dysplasias and oral squamous cell carcinoma: A diagnostic marker for carcinogenesis. J Oral Maxillofac Pathol. 2018 Jan-Apr; 22(1):59-64. 10. Solomon MC, Carnelio S, Gudattu V. Molecular analysis of oral squamous cell carcinoma: A tissue microarray study. Indian J Cancer. 2010; 47:166–72. 11. Smilek P, Dusek L, Vesely K, Rottenberg J, Kostrica R. Correlation of expression of Ki67, EFGR, C-erbB2, MMP-9, p53, bcl2, CD34 and cell cycle analysis with survival in head and neck squamous cell cancer. J Exp Clin Cancer Res. 2006; 25:549–55. 12. Fregonesi PA, Teresa DB, Duarte RA, Neto CB, Oliveira M, Soares CP. p16ink4a immunohistochemical overexpression in premalignant and malignant oral lesions infected with human papillomavirus. J Histochem Cytochem. 2010; 51:1291–97. 13. 13.Yuen AP, Lam KY, Choy JT, Ho WK, Wong LY, Wei WI. Clinicopathologic significance of bcl-2 expression in the surgical treatment of oral squamous cell carcinoma. J Surg Oncol. 2002; 94:713–22. 14. Ai L et al. The p16 (CDKN2a/INK4a) tumorsuppressor gene in head and neck squamous cell carcinoma: A promoter methylation and protein expression study in 100 cases. Mod Pathol. 2003; 16:944–50. 15. Ralli M, Singh S, Yadav SP, Sharma N, Verma R, Sen R. Assessment and clinicopathological correlation of p16 expression in head and neck squamous cell carcinoma. J Cancer Res Ther. 2016; 12:232–7.