Published November 30, 2023 | Version https://impactfactor.org/PDF/IJPCR/15/IJPCR,Vol15,Issue11,Article225.pdf
Journal article Open

A Study of Bacteriological Profile of Pus in a Tertiary Care Hospital in Western UP

  • 1. M.D. (Microbiology), Associate Professor, Microbiology, Rama Medical College Hospital & Research Centre, Hapur, U.P, India
  • 2. M.Sc. (Biotechnology), Department of Biotechnology, Multanimal Modi College, Ghaziabad, U.P, India
  • 3. M.D. (Microbiology), Professor & HOD, Microbiology, Rama Medical College Hospital & Research Centre, Hapur, U.P, India
  • 4. M.Sc. (Biotechnology), Lecturer, Biotechnology, Dr. Kedarnath Modi Institute of Pharmaceutical Education and Research, Ghaziabad, U.P, India

Description

Introduction: Pus formation is typically caused by a bacterial infection. Surgical Site Infection (SSI) is one of the most common causes of nosocomial infections. Resistance to commonly prescribed antibiotics for pus infection is an expanding global problem. So, this study was done to determine the prevalence of pus infection and bacterial profile of organism causing pus infection. Material and Methods: All samples were processed by standard microbiological procedures including Aerobic culture, Morphology, Gram Stain, Motility, Biochemical tests and Antimicrobial susceptibility test. Results: Culture positive pus samples were found to be 90.7%. There were 43% Gram positive cocci and 57% Gram negative bacilli isolated. Klebsiella species was found to be most common. Conclusion: Increasing multidrug resistance in pus infections is an important and emerging public health problem. The empirical treatment guidelines must be adjusted accordingly.

 

 

Abstract (English)

Introduction: Pus formation is typically caused by a bacterial infection. Surgical Site Infection (SSI) is one of the most common causes of nosocomial infections. Resistance to commonly prescribed antibiotics for pus infection is an expanding global problem. So, this study was done to determine the prevalence of pus infection and bacterial profile of organism causing pus infection. Material and Methods: All samples were processed by standard microbiological procedures including Aerobic culture, Morphology, Gram Stain, Motility, Biochemical tests and Antimicrobial susceptibility test. Results: Culture positive pus samples were found to be 90.7%. There were 43% Gram positive cocci and 57% Gram negative bacilli isolated. Klebsiella species was found to be most common. Conclusion: Increasing multidrug resistance in pus infections is an important and emerging public health problem. The empirical treatment guidelines must be adjusted accordingly.

 

 

Files

IJPCR,Vol15,Issue11,Article225.pdf

Files (655.4 kB)

Name Size Download all
md5:a2784181912b8e86b0b72377100d398f
655.4 kB Preview Download

Additional details

Dates

Accepted
2023-10-30

References

  • 1. Tsuchida Y, Hayashi R, Ansai O, Nakajima M, Oginezawa M, Kawai T, Yokoyama R, Deguchi T, Hama N, Shinkuma S, Abe R. Generalized pustular psoriasis complicated with bullous pemphigoid. Int J Dermatol. 2019 Mar;58(3):e66-e67. 2. Negi V, Pal S, Juyal D, Sharma MK, Sharma N. Bacteriological Profile of Surgical Site Infections and Their Antibiogram: A Study From Resource Constrained Rural Setting of Uttarakhand State, India. J ClinDiagn Res. 2015 Oct;9(10):DC17-20. 3. Hohmann C, Eickhoff C, Radziwill R, Schulz M. Adherence to guidelines for antibiotic prophylaxis in surgery patients in German hospitals: a multicentre evaluation involving pharmacy interns. Infection. 2012 Apr;40(2):131-7. 4. Pradhan GB, Agrawal J. Comparative study of post-operative wound infection followingemergency lower segment caesarean section with and without the topical use of fusidic acid. Nepal Med Coll J. 2009 Sep;11(3):189- 91. 5. Owens CD, Stoessel K. Surgical site infections: epidemiology, microbiology and prevention. J Hosp Infect. 2008 Nov;70 Suppl 2:3-10. 6. Aggarwal S, Jena S, Panda S, Sharma S, Dhawan B, Nath G, Singh NP, Nayak KC, Singh DV. Antibiotic Susceptibility, Virulence Pattern, and Typing of Staphylococcus aureus Strains Isolated from Variety of Infections in India. Front Microbiol. 2019 Dec 4;10:2763. 7. Clinical and Laboratory Standards Institute, 2022. Performance standards for antimicrobial susceptibility testing; CLSI Publication M100- Ed32 8. Khanam RA, Islam MR, Sharif A, Parveen R, Sharmin I, Yusuf MA. Bacteriological Profiles of Pus with Antimicrobial Sensitivity Pattern at a Teaching Hospital in Dhaka City. Bangladesh Journal of Infectious Diseases 2018. 5. 9. Swati Duggal, P K Khatri, R S Parihar, RajatArora. Antibiogram of Various Bacterial Isolates from Pus Samples in a Tertiary Care Centre in RajasthanInt J Sci Res. 2015;45:1580-84 10. Mary Shama, KulandhaivelMurugesan, HridhyaVijayan. Isolation Identification and Antibiotic Sensitivity Pattern of Pyogens from Pyogenic PathogensBiomed Pharmacol20181114638 11. Mantravadi HB, Chinthaparthi MR, Shravani V. Aerobic isolates in pus and their antibiotic sensitivity pattern: a study conducted in a teaching hospital in Andhra Pradesh. Int J Med Sci Public Health 2015; 4:1076-1079 12. Tiwari HK, Das AK, Sapkota D, Sivrajan K, Pahwa VK. Methicillin resistant Staphylococcus aureus: prevalence and antibiogram in a tertiary care hospital in western Nepal. J Infect DevCtries. 2009 Oct 22;3(9):681-4. 13. Rai S, Yadav UN, Pant ND, Yakha JK, Tripathi PP, Poudel A, Lekhak B. Bacteriological Profile and Antimicrobial Susceptibility Patterns of Bacteria Isolated from Pus/Wound Swab Samples from Children Attending a Tertiary Care Hospital in Kathmandu, Nepal. Int J Microbiol. 2017; 2017:2529085. 14. Adhikari R, Pant ND, Neupane S, Neupane M, Bhattarai R, Bhatta S, Chaudhary R, Lekhak B. Detection of Methicillin Resistant Staphylococcus aureus and Determination of Minimum Inhibitory Concentration of Vancomycin for Staphylococcus aureus Isolated from Pus/Wound Swab Samples of the Patients Attending a Tertiary Care Hospital in Kathmandu, Nepal. Can J Infect Dis Med Microbiol. 2017; 2017:2191532. 15. Trojan R, Razdan L, Singh N. Antibiotic Susceptibility Patterns of Bacterial Isolates from Pus Samples in a Tertiary Care Hospital of Punjab, India. Int J Microbiol. 2016; 2016:9302692. 16. I. Garba, Y.H. Lusa, E. Bawa, M.B. Tijjani, M.S. Aliyu, U.U. Zango and M.I.O. Raji. Antibiotics Susceptibility Pattern of Pseudomonas aeruginosa Isolated from Wounds in Patients Attending Ahmadu Bello University Teaching Hospital, Zaria, Nigeria. Nigerian Journal of Basic and Applied Science (March 2012), 20(1): 32-34.