PARAGON 2 - KM2209 - Particulate Carbon and Nitrogen Timecourse Incubations
Description
This dataset contains measurements of particulate carbon and nitrogen concentrations collected during the PARAGON 2 expedition (KM2209) in the North Pacific Subtropical Gyre. Measurements come from time-course incubation experiments initiated with whole seawater collected at 150 m using trace metal clean techniques and modified with various additions of organic carbon, iron, and/or nitrogen. Samples for measurements of particulate carbon and nitrogen concentrations were collected at multiple time points for each experimental biological replicate by filtering 4 L of seawater from polycarbonate incubation bottles onto pre-combusted 25 mm glass fiber filters (GF/F, Whatman) under positive pressure. Filters were then transferred to polystyrene Petri dishes lined with pre-combusted aluminum foil and stored at -20°C until analysis onshore. Filters were then analyzed by high temperature combustion using an Exeter CE-440 Elemental Analyzer according to Grabowski et al. (2019). Particulate carbon concentrations are measurements of total particulate carbon, including both organic and inorganic carbon. Timestamp is in UTC.
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Additional details
Funding
- Simons Foundation
- Simons Collaboration on Ocean Processes and Ecology 721221
- Simons Foundation
- Simons Postdoctoral Fellowship in Marine Microbial Ecology 729162
Dates
- Collected
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2022-08
References
- Grabowski E, Letelier RM, Laws EA, Karl DM. Coupling carbon and energy fluxes in the North Pacific Subtropical Gyre. Nat Commun. 2019;10(1). https://doi.org/10.1038/s41467-019-09772-z