Published January 30, 2024 | Version https://impactfactor.org/PDF/IJPCR/16/IJPCR,Vol16,Issue1,Article299.pdf
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Identification of Candida Species from Different Respiratory Samples by Using the Phenotypic Method in a Tertiary Care Centre

  • 1. Assistant professor, Department of Microbiology, Mahatma Gandhi Memorial Medical College, Indore, Madhya Pradesh
  • 2. PG Resident, Department of Microbiology, Mahatma Gandhi Memorial Medical College, Indore, Madhya Pradesh
  • 3. Professor, Department of Microbiology, Mahatma Gandhi Memorial Medical College, Indore, Madhya Pradesh
  • 4. Associate Professor, Department of Microbiology, Mahatma Gandhi Memorial Medical College, Indore, Madhya Pradesh

Description

Background and Objective: In recent years, the incidence of Candidiasis has witnessed a concerning upsurge, resulting in a significant healthcare challenge. These infections are further enhanced by factors like the widespread use of broad-spectrum antimicrobials, chemotherapy-induced neutropenia, and the presence of medical devices. One factor that may be crucial in limiting disseminated candidiasis is the colonisation of Candida species in the respiratory systems of susceptible hosts. This study was designed to identify Candida species in all respiratory samples. Material Method: Sampling was conducted from 2021 to 2023. A total of 86 clinical isolates of Candida species were obtained from the respiratory samples of both immunocompromised and immunocompetent patients. The samples were initially inoculated on SDA and examined under a KOH mount. The growth was identified using conventional microbiological techniques. Result: In our study, Candida albicans (n=45 / %=54.65) was most isolated, followed by Candida tropicalis (n=26 / %=32.55and then Candida krusei (n=5 / %=8.13), Candida dublinensis (n=1 / %=2.32), and Candida glabrata (n=1 / %=2.32). In our study, 30–50 years is the most common affected age group. Conclusion: In recent decades, non-Albicans candida has become more widely reported. Early and accurate diagnosis is very essential for the successful management of patients.

 

 

Abstract (English)

Background and Objective: In recent years, the incidence of Candidiasis has witnessed a concerning upsurge, resulting in a significant healthcare challenge. These infections are further enhanced by factors like the widespread use of broad-spectrum antimicrobials, chemotherapy-induced neutropenia, and the presence of medical devices. One factor that may be crucial in limiting disseminated candidiasis is the colonisation of Candida species in the respiratory systems of susceptible hosts. This study was designed to identify Candida species in all respiratory samples. Material Method: Sampling was conducted from 2021 to 2023. A total of 86 clinical isolates of Candida species were obtained from the respiratory samples of both immunocompromised and immunocompetent patients. The samples were initially inoculated on SDA and examined under a KOH mount. The growth was identified using conventional microbiological techniques. Result: In our study, Candida albicans (n=45 / %=54.65) was most isolated, followed by Candida tropicalis (n=26 / %=32.55and then Candida krusei (n=5 / %=8.13), Candida dublinensis (n=1 / %=2.32), and Candida glabrata (n=1 / %=2.32). In our study, 30–50 years is the most common affected age group. Conclusion: In recent decades, non-Albicans candida has become more widely reported. Early and accurate diagnosis is very essential for the successful management of patients.

 

 

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Dates

Accepted
2023-12-26

References

  • 1. Murray PR, Van Scoy RE, Roberts GD. Should yeasts in respiratory secretions be identified? Mayo Clin Proc. 1977; 52:42–45. 2. Pradeep Reddy Anam, Ved Prakash, Deepika Verma and Ramesh Babu Myneni, Prevalence of Candida species and their Susceptibility to Triazoles in Clinical Isolates from a Tertiary Care Hospital, J Pure Appl Microbiol. 2023; 17(4):2437-2442. doi: 10.22207/ JPAM. 17. 4.41. 3. Al., R. A. K. et. Characterisation and antifungal susceptibilty testing for candida species in a tertiary care hospital. J. Heal. Sci. Res.2, (2011). 4. B, V. K., G, A. K., Swapna, M. and Easow, J. M. Isolation and identification of candida species from various clinical samples in a tertiary care hospital. 5, 3520–3522 (2017) 5. Athira Jayaram, Khushboo Sareen, Ashiwini Dedwal, Sushma Pednekar, Sunil Bhamare, Swati Mulshingkar, Rajesh Karyakarte, Mycological Profile of Respiratory Tract Samples in a Tertiary Care Hospital from Western India, International Journal of Health Sciences and Research, Vol.11; Issue: 8; August 2021. 6. Durga Shankar Meena Deepak Kumar, Candida Pneumonia: An Innocent Bystander or a Silent Killer? Brief Report,Med Princ Pract 2022;31:98–102., 7. Montes K, Ortiz B, Galindo C, Figueroa I, Braham S, Fontecha G (2019) Identifcation of Candida species from clinical samples in a Honduran Tertiary Hospital. Pathogens 8(4):237. https://doi.org/10.3390/ pathogens 8040237. 8. Sanglard, D. Emerging Threats in AntifungalResistant Fungal Pathogens. Front. Med. (Lausanne) 2016, 3, 11. [CrossRef] 9. Meersseman W, Lagrou K, Spriet I et al. Significance of the isolation of Candida species from airway samples in critically ill patients: a prospective, autopsy study. Intensive Care Med 2009; 35:1526–31. 10. Benjamin J. Moss, and Daniel M. Musher, Candida species in community-acquired pneumonia in patients with chronic aspiration, Moss and Musher Pneumonia, (2021) 13:12. 11. Athira Jayaram, Khushboo Sareen, Ashiwini Dedwal, Sushma Pednekar, Sunil Bhamare, Swati Mulshingkar, Rajesh Karyakarte, Mycological Profile of Respiratory Tract Samples in a Tertiary Care Hospital from Western India, International Journal of Health Sciences and Research, Vol.11; Issue: 8; August 2021. 12. Oveimar De La Cruz, MDa, Fernanda P. Silveira, MD, MS, Respiratory Fungal Infections in Solid Organ and Hematopoietic Stem Cell Transplantation, Clin Chest Med 38 (2017) 727–739. 13. Mona M. Ahmeda, Ayman A. Farghalyb, Riham H. Raafata, Waleed M. Abd Elsattar, Study of the prevalence and pattern of fungal pneumonias in respiratory intensive care units, Egyptian Journal of Bronchology, Vol. 13 No. 4, October-December 2019. 14. Mojtaba Taghizadeh Armaki1(Ph.D student), Mohammad Taghi Hedayati2٭(Ph.D), Saeed Mahdavi Omran3(Ph.D), Sasan Saber4(MD), Mahdi Abastabar2(Ph.D), Akbar Hosseinnejad1(MSc Student), Identification and Antifungal Susceptibility Testing of Candida species isolated from Bronchoalveolar Lavage samples, International Journal of Molecular and Clinical Microbiology, y 1 (2014) 358- 364. 15. Mohammad Khairy El-Badrawy, Amany Ragab Elsaied, Asmaa Adel Metwally Ibrahim, Ahmed Elsayed Eladl and Rehab Ahmad Elmorsey, Prevalence and pattern of isolated fungi from bronchoalveolar lavage among patients with lung cancer: a prospective cross-sectional study, The Egyptian Journal of Bronchology, (2023)17:7 16. Golia S, Reddy KM, Karjigi KS, Hittinahalli V. Speciation of Candida using chromogenic and cornmeal agar with determination of fluconazole sensitivity. Al Ameen J Med Sci. 2013; 6(2):163-6. 17. Vijaya D, Harsha TR, Nagaratanamma T. Candidaspeciation using CHROM agar. J Clin Diagn Res. 2011; 5(4):755-7. 18. Adhikari R, Joshi S. Species distribution and anti-fungal susceptibility of candidemiaat a multi super-speciality center in Southern India. Ind J Med Microbiol. 2013; 29:309-11.