A Study on Time Related Changes in Bacterial Pattern in Burn Wound Infections and Their Antibiogram in a Tertiary Care Hospital
Creators
- 1. Professor & Head, Department of Microbiology, Kurnool Medical College Kurnool
- 2. Associate Professor, Department of Microbiology, Kurnool Medical College Kurnool
- 3. Assistant Professor, Department of Microbiology, Kurnool Medical College Kurnool
- 4. Senior Resident, Department of Microbiology, Kurnool Medical College Kurnool
Description
Background: Burns remain a significant health problem in terms of morbidity, long term disability & mortality throughout the world, especially in economically developing countries. Objectives: To identify the bacterial agents responsible for burn wound infections from admission day 0 to day 21 and to study the antibiogram of bacterial isolates for effective infection control. And also to detect various resistant organisms like MRSA, ESBL, AmpC β-lactamase and MBL producers phenotypically. Results: 112 burn patients were included in the present study. A total of 448 swabs were collected. Most frequent isolate was Pseudomonas aeruginosa 30.15%, followed by Staphylococcus aureus 23.71%, Klebsiella pneumoniae 12.3%, Coagulase negative Staphylococci 10.82%, Escherichia coli 9.27%, Acinetobacter baumanii 6.95%. MRSA was isolated in 30.43% cases. ESBL, AmpC, MBL production was seen in 16%, 8% and 10% cases respectively. Studying the time related changes of bacteria in burns wound showed that on day 0, most of the samples were sterile and an initial predominance of Gram positive cocci in the first week. From day 14, Gram negative bacilli began to predominate. Antimicrobial sensitivity testing showed Colistin to be very effective drugs for Gram negative bacilli while Linezolid very effective for Gram positive isolates. Conclusion: The antimicrobial treatment must be changed as microbial flora of the burn wound is an ever-changing entity. Constant analysis of the wound cultures will help the treating physicians to keep abreast with the pathogens and their antimicrobial susceptibility. Early detection of the ESBL, AmpC, and MBL producing isolates in a diagnostic laboratory could help to avoid treatment failure. Aggressive infection control measures should be applied to limit the emergence and spread of these pathogens.
Abstract (English)
Background: Burns remain a significant health problem in terms of morbidity, long term disability & mortality throughout the world, especially in economically developing countries. Objectives: To identify the bacterial agents responsible for burn wound infections from admission day 0 to day 21 and to study the antibiogram of bacterial isolates for effective infection control. And also to detect various resistant organisms like MRSA, ESBL, AmpC β-lactamase and MBL producers phenotypically. Results: 112 burn patients were included in the present study. A total of 448 swabs were collected. Most frequent isolate was Pseudomonas aeruginosa 30.15%, followed by Staphylococcus aureus 23.71%, Klebsiella pneumoniae 12.3%, Coagulase negative Staphylococci 10.82%, Escherichia coli 9.27%, Acinetobacter baumanii 6.95%. MRSA was isolated in 30.43% cases. ESBL, AmpC, MBL production was seen in 16%, 8% and 10% cases respectively. Studying the time related changes of bacteria in burns wound showed that on day 0, most of the samples were sterile and an initial predominance of Gram positive cocci in the first week. From day 14, Gram negative bacilli began to predominate. Antimicrobial sensitivity testing showed Colistin to be very effective drugs for Gram negative bacilli while Linezolid very effective for Gram positive isolates. Conclusion: The antimicrobial treatment must be changed as microbial flora of the burn wound is an ever-changing entity. Constant analysis of the wound cultures will help the treating physicians to keep abreast with the pathogens and their antimicrobial susceptibility. Early detection of the ESBL, AmpC, and MBL producing isolates in a diagnostic laboratory could help to avoid treatment failure. Aggressive infection control measures should be applied to limit the emergence and spread of these pathogens.
Files
IJPCR,Vol16,Issue1,Article258.pdf
Files
(692.9 kB)
| Name | Size | Download all |
|---|---|---|
|
md5:2847ca32e5a133970b038c40a2a329c1
|
692.9 kB | Preview Download |
Additional details
Dates
- Accepted
-
2023-12-26
Software
- Repository URL
- https://impactfactor.org/PDF/IJPCR/16/IJPCR,Vol16,Issue1,Article258.pdf
- Development Status
- Active
References
- 1. Weeks BS. Brief introduction to the history of burns medical science. Burns Regenerative Medicine and Therapy 2000:1-3. Available from URL:http:// www.ele.uri.edu/ courses/bme281/F12/CaraN_1.pdf 2. Mayhall CG. The epidemiology of burn wound infections: Then and now. Clin Infect Dis. 2003; 37:543-50. 3. Mohan H. Textbook of pathology. 4th Ed. Delhi: Jaypee Brothers Medical Publishers (P) Ltd, 2000. 4. Artz CP. Historical aspects of burn management. Surg Clin North Am. 1970:50:1193- 1200. 5. Atiyeh BS, Al-Amm CA. Immunology of burn injury - an overview. Ann Bur Fire Disasters. 2001;14(2):78-84. 6. Cruickshank R. The bacterial infection of burns. J Path and Bact. 1935:4:367369. 7. Kaur H, Bhat J, Anvikar AR, Rao S, Gadge V. Bacterial profile of blood & burn wound infections in burn patients. In: Proceedings of National Symposium on Tribal Health; 2006 Oct 19-20; Jabalpur, India: 89-95. 8. Ramakrishnan MK, Sankar J, Venkatraman J, Ramesh J. Infection in burn patients- experience in a tertiary care hospital. Burns. 2006; 32-594-596. 9. Jefferson Lessa Soares de Macedo, Joao Barberino Santos. Bacterial and fungal colonization of burn wounds, Mem Inst Oswaldo Cruz, Rio de Janeiro. 2005;100(5):535- 539. 10. Mehta M, Dutta P, Gupta V. Bacterial isolates from the burn wound infections & their antibiograms: A eight-year study. Indian J Plast Surg. 2007;40(1):25-28. 11. Kaushik R, Kumar S, Sharma R, Lal P. Bac-teriology of burn wounds - the first three years in a new burns unit at the medical College Chandigarh. Burns. 2001:27:595-597. 12. Rajput A. Singh KP, Kumar V, Sexena R, Singh RK. Antibacterial resistance patternof nerobic bacteria isolates from burn patients in tertiary care hospital Biomed Res. 2008; 19(1):1-4. 13. Angus Nnamdi Oli, Dennis Emeka Eze, Thaddeus Harrison Gugu, Ifeanyi Ezeobi, Ukamaka Nwakaku Maduagwu, Chibueze Peter Ihekwereme Pan Afr Med. J. 2017; 27: 66. 14. Gupta AK, Uppal S, Garg R, Gupta A. Pal R. A clinic-epidemiologic study of 892 patients with burn injuries at a tertiary care hospital in Punjab, India. JEmerg Trauma Shock. 2011; 4:7-11. 15. Sengupta SR, Bansal MP, Destipende PK, Sharma KD. Infectiim of turns Ind J Surg 1972; 34(11):427-432 31. Saboo RM. Agarwal SC, Balakrishnan C. Pattern of infection in burn. Ind Surg. 1984:3:289-294.