Published April 29, 2024 | Version v1
Journal article Open

Uncovering the hidden diversity of non-biting midges (Diptera, Chironomidae) from central Namibia, using morphology and DNA barcodes

  • 1. Estación Biológica de Doñana-CSIC/Doñana Biological Station-CSIC, Sevilla, Spain
  • 2. Shanghai Ocean University, Shanghai, China
  • 3. Bavarian State Collection of Zoology (SNSB-ZSM), Munich, Germany

Description

The first study results on the Chironomidae fauna of central Namibia (Khomas, Otjozondjupa and Hardap regions) are presented, based on morphology and DNA-barcoding. The preliminary investigation led to the discovery of a new species Paraphaenocladius namibiae sp. nov. (Chironomidae, Orthocladiinae) and 17 new country records for Namibia.

Files

AI_article_111920.pdf

Files (11.5 MB)

Name Size Download all
md5:905360a778038e2c0df20e05451f9ba0
11.5 MB Preview Download

System files (182.4 kB)

Name Size Download all
md5:fb30d7ed992a419e79fe692e220bc1cb
182.4 kB Download

Linked records

Additional details

References

  • Andersen T, Mendes H (2010) Order Diptera, family Chironomidae (with the exception of the tribe Tanytarsini). Arthropod fauna of the UAE 3: 564–598.
  • Ashe P, O'Connor JP (2009) A World Catalogue of Chironomidae (Diptera). Part 1. Buchonomyiinae, Chilenomyiinae, Podonominae, Aphroteniinae, Tanypodinae, Usambaromyiinae, Diamesinae, Prodiamesinae and Telmatogetoninae. Irish Biogeographical Society/The National Museum of Ireland, Dublin, 445 pp.
  • Ashe P, O'Connor JP (2012) A world catalogue of Chironomidae (Díptera). Part 2. Orthocladiinae. Two volumes (Sections A, B). Irish Biogeographical Society, Dublin, [14] + [xvi +] 468 pp., [6] + 500 pp.
  • Curtis B (1991) Freshwater macro-invertebrates of Namibia. Madoqua 1991: 163–187.
  • Cranston PS, Judd DD (1989) Diptera: Family Chirononlidae of the Arabian Peninsula. Fauna Saudi Arabia 10: 236–289.
  • Dejoux C (1968a) Contribution à l'étude des premiers états des Chironomides du Tchad Hydrobiologia 31: 449–464. https://doi.org/10.1007/BF00134446
  • Dejoux C (1968b) Le Lac Tchad et les Chironomides de sa partie Est. Annales Zoologici Fennici 5: 27–32.
  • Dejoux C (1974) Contribution à la connaissance des Chironomides de l'Afrique de l'Ouest. Entomologisk tidskrift 95: 72–83.
  • Ekrem T (2001) A review of Afrotropical Tanytarsus van der Wulp (Diptera: Chironomidae). Tijdschrift voor Entomologie 144(1): 5–40. https://doi.org/10.1163/22119434-99900054
  • Ekrem T, Ashe P, Andersen T, Stur E (2017) Chapter 35. Chironomidae (non-biting midges). In: Kirk-Spriggs AH, Sinclair BJ (Eds) Manual of Afrotropical Diptera. Vol. 2. Nematocerous Diptera and lower Brachycera. Suricata 5. SANBI Graphics and Editing, Pretoria, 813–863.
  • Ferrington Jr LC, Sæther OA (2011) A revision of the genera Pseudosmittia Edwards, 1932, Allocladius Kieffer, 1913, and Hydrosmittia gen. n. (Diptera: Chironomidae, Orthocladiinae). Zootaxa 2849(1): 1–314. https://doi.org/10.11646/zootaxa.2849.1.1
  • Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3: 294–299.
  • Freeman P (1953) Chironomidae (Diptera) from Western Cape province-I. Proceedings of the Royal Entomological Society of London. Series B, Taxonomy 22(7-8): 127–135. https://doi.org/10.1111/j.1365-3113.1953.tb00071.x
  • Freeman P (1955a) A study of the Chironomidae (Diptera) of Africa south of the Sahara. Part I. Bulletin of the British Museum (Natural History). Historical Series 4: 1–67. [Natural History]
  • Freeman P (1955b) A study of the Chironomidae (Diptera) of Africa south of the Sahara. Part IV. Bull. Br. Mus.(Natl. Hist.). Entomol. 6: 263–363. https://doi.org/10.5962/bhl.part.17110
  • Freeman P (1956) A study of the Chironomidae (Diptera) of Africa south of the Sahara. Part II. Bulletin of the British Museum (Natural History). Entomology 4: 285–368.
  • Freeman P (1957) A study of the Chironomidae (Diptera) of Africa South of the Sahara. Part III. Bulletin of the British Museum (Natural History). Entomology 5: 321–428. https://doi.org/10.5962/bhl.part.1515
  • Freeman P (1958) A study of the Chironomidae (Diptera) of Africa South of the Sahara. Part IV. Bulletin of the British Museum (Natural History). Entomology 6(11): 26–364. https://doi.org/10.5962/bhl.part.17110
  • Freeman P (1961) A collection of Chironomidae and Culicidae subfamily Dixinae (Diptera, Nematocera) from Madagascar. Memoires de l'Institut Scientifique de Madagascar. Serie E, Entomologie 12: 238–255.
  • Freeman P, Cranston P (1980) Family Chironomidae. Catalogue of the Diptera of the Afrotropical Region. British Museum (Natural History), London: 175–202.Ghonaim, M., Ali, A., & Amer, M. (2005). Cladotanytarsus (Diptera: Chironomidae) from Egypt with description of a new species. Oriental Insects 39(1): 323–329. https://doi.org/10.1080/00305316.2005.10417443
  • Gilka W (2009) Order Diptera, family Chironomidae, Tribe Tanytarsini. In: van Harten A (Ed.) Arthropod Fauna of the United Arab Emirates. Dar Al Ummah Print, Abu Dhabi, 667–682.
  • Gratton C, Donaldson J, Zanden MJV (2008) Ecosystem Linkages Between Lakes and the Surrounding Terrestrial Landscape in Northeast Iceland. Ecosystems (New York, N.Y. ) 11(5): 764–774. https://doi.org/10.1007/s10021-008-9158-8
  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Oxford, 95–98.
  • Harrison A (2004) The non-biting midges (Diptera: Chironomidae) of South Africa. University of Waterloo, Canada. http://www.ru.ac.za/academic/departments/zooento/Martin/chironomidae
  • Haug JT, Haug C, Ehrlich M (2008) First fossil stomatopod larva (Arthropoda: Crustacea) and a new way of documenting Solnhofen fossils (Upper Jurassic, Southern Germany). Palaeodiversity 1: 103–109.
  • Hebert PD, Braukmann TW, Prosser SW, Ratnasingham S, DeWaard JR, Ivanova NV, Janzen DH, Hallwachs W, Naik S, Sones JE, Zakharov EV (2018) A Sequel to Sanger: Amplicon sequencing that scales. BMC Genomics 19(1): 1–14. https://doi.org/10.1186/s12864-018-4611-3
  • Ivanova NV, Grainger C (2007) Primer Sets for DNA Barcoding.
  • Kirk-Spriggs AH (2017) Chapter 2. Collection and preservation of Diptera. In: Kirk-Spriggs AH, Sinclair BJ (Eds) Volume 1: Introductory chapters and keys to Diptera. Suricata 4. South African National Biodiversity Institute, Pretoria, 69–87.
  • Lin X-L, Stur E, Ekrem T (2018) DNA barcodes and morphology reveal unrecognized species in Chironomidae (Diptera). Insect Systematics & Evolution 49(4): 329–398. https://doi.org/10.1163/1876312X-00002172
  • Lindner E (1976) Zur Kenntnis der Dipterenfauna Sudwestafrikas. VI VIII. Journa of South West African Scientific Society 30: 75–86.
  • Martin J (1999) Listing of names which have been applied in the genus Kiefferulus Goetghebuer. https://www.chironomidae.net/Martin/Chironfiles/Kiefffile/Kieff.htm [Accessed: 15/12/2023]
  • Qi X, Lin XL, Wang XH (2012) Review of Dicrotendipes kieffer from China (Diptera, Chironomidae). ZooKeys 183: 23–26. https://doi.org/10.3897/zookeys.183.2834
  • Ratnasingham S, Hebert PD (2007) BOLD: The Barcode of Life Data System (http://www.barcodinglife.org). Molecular Ecology Notes 7(3): 355–364. https://doi.org/10.1111/j.1471-8286.2007.01678.x
  • Reid H, Sahlén L, Stage J, MacGregor J (2007) The economic impact of climate change in Namibia. IIED, London, 46 pp.
  • Sæther OA (1980) Glossary of chironomid morphology terminology (Diptera: Chironomidae). Entomologica Scandinavica (Supplement 14): 1–51.
  • Sæther OA, Wang X (1995) Revision of the genus Paraphaenocladius Thienemann, 1924 of the world (Diptera: Chironomidae, Orthocladiinae). Entomologica scandinavica supplement 48: 1–69.
  • Thienemann A, Zavřel J 1916. Die Metamorphose der Tanypinen. Archiv für Hydrobiologie - Supplements 2(3): 566–654.
  • Wang XH, Sæther OA, Andersen T (2002) Afrotropical Bryophaenocladius Thienemann, 1934 (Diptera: Chironomidae). Studia Dipterologica 8: 447–462.
  • Zinchenko TD, Gladyshev MI, Makhutova ON, Sushchik NN, Kalachova GS, Golovatyuk LV (2014) Saline rivers provide arid landscapes with a considerable amount of biochemically valuable production of chironomid (Diptera) larvae. Hydrobiologia 722(1): 115–128. https://doi.org/10.1007/s10750-013-1684-5