PROSPECTS FOR USE OF SUPRAMOLECULAR COMPLEXES OF MONOAMMONIUM GLYCYRRHIZINATE WITH POLYPHENOLS AND AMINO ACIDS AS THE HEPATOPROTECTORS

Supramolecular complexes of the monoammonium glycyrrhizinate (MG) with amino acids, to name methionine (Met) and DL-carnitine-HCL (Car) and phenolic compounds, to name quercetin (Que) and methoxycinnamic acid (MethA) in molar ratio 4:1, were generated as promising low-dose cytoprotectors/hepatoprotectors. After induction of acetaminophen-induced hepatitis (1500 mg/kg for 2 days intragastrically) and the 7-day administration of complexes, MG/Met and MG/MethA caused statistically significant shortening of duration of hexobarbital-induced sleep at the dose of 2,5 mg/kg (160±14,0 min in control vs 36,1±2,3 and 33,4±2,2 min, respectively, p<0,05 both) and duration of thiopental-induced sleep at the dose of 5 mg/kg (63,5±4,2 min in control vs 20,7±1,6 and 17,7±1,2 min, respectively, p<0,05 both); MG/Car and MG/Que at the dose 5 mg/kg caused the most significant reduction in activity of the enzyme alkaline phosphatase (245,2±16,4 U/l in control vs 88,33±4,67 and 96,13±4,27 U/l, respectively, p<0,05 both). According to histological studies MG/Met and MG/MethA at the dose of 5 mg/kg caused more significant reduction in the dyscirculatory and edematous events; signs of activation of lymphoid and macrophagal cells in the liver tissue appeared. Under normal physiological conditions, the complexes under study were not found to produce any effects on the bile secreting function of the liver. These findings demonstrate necessity to generate a complex medication combining various effects indispensable for recovery of various functions of the liver after acute injuries, toxic effects of xenobiotics, as well as for prevention of grave complications of hepatitis