Published April 19, 2024 | Version 0
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Oregon Wolfe Barley (Hordeum vulgare) Informative & Spectacular Subset (ISS) vegetative stage growth data

  • 1. ROR icon Purdue University West Lafayette


Oregon Wolfe Barley Informative & Spectacular Subset (ISS) was raised at the Ag Alumni Seed Phenotyping Facility (AAPF) at Purdue University (West Lafayette, Indiana, USA) for 42 days. There were 18 genotypes, with two replicates for each genotype (total plants: 36). AAPF is a controlled environment high-throughput phenotyping facility with automated imaging and irrigation systems. A virtual tour of AAPF can be found at

Seeds were sown in a 6 L pot with 2.8 L of Profile Porous Ceramic Greens Grade and Berger BM6 each with 10g of Osmocote. Five hundred ml of Turface was laid on top of each pot to avoid effect of algae for RGB data derivation. The growth temperature in the chamber was 72/68 degrees Fahrenheit day/night. Relative humidity was set at 60%. Lighting was 16 h day/8 h night.

Plants were imaged with RGB camera from one top and 12 side views three times a week, ranging between 10 days from planting (equivalent to sowing, Dfp) to 42 Dfp. Ground reference data of plant height and tiller count were measured twice a week. 


RGB imaging data were stored in “OWB_RGB.xlsx”. Datasheet “Information” describes the variables in datasheets for top view, side average view and every side view.


Ground reference data for plant height and tiller count were stored in “OWB_ground_reference.xlsx”. Datasheet “Information” describes the variables in datasheet “Data”.


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