Antimicrobial Susceptibility Pattern of E. Coli Causing Urinary Tract Infection with Special Reference to Fluoroquinolone Resistance in a Tertiary Care Teaching Hospital in South India
Authors/Creators
- 1. Associate Professor, Department of Microbiology, Trichy SRM Medical College Hospital and Research Centre, Trichy, Tamilnadu, India (formerly known as Chennai Medical College Hospital and Research Centre, Trichy)
- 2. Professor, Department of Microbiology, Trichy SRM Medical College Hospital and Research Centre, Trichy, Tamilnadu, India
- 3. Research Faculty, Department of Research, Department of Microbiology, Trichy SRM Medical College Hospital and Research Centre, Trichy, Tamilnadu, India
Description
Background: Antimicrobial resistance among Escherichia coli causing urinary tract infection (UTI) is a public health concern. Objectives: 1. To find the prevalence of E. coli causing urinary tract infections from urine samples and to determine the antimicrobial susceptibility pattern of E. coli isolates by Kirby Bauer’s disc diffusion method. 2. To study about the minimum inhibitory concentration (MIC) fluoroquinolone resistant E. coli isolates by Microbroth dilution method. Method: The Prospective study was conducted among 150 UTI Patients attending Tertiary care centre for treatment. Ethical principles were adhered. Their socio – demographic details were collected and received urine samples were processed using standard methods and antibiotic susceptibility was done by Kirby-Bauer disk diffusion test and minimum inhibitory concentration of fluoroquinolone resistant E. coli isolates by Microbroth dilution method. Data was analysed using SPSS Software version 23.0. Results: The mean age of the participants was 48.55 + 14.37. Females were predominant. Majority, 49.3% belonged to 31-60 years age group. Among the 241 isolated organisms from culture, the most common were E. coli 62.2% (150) and Klebsiella pneumoniae was found to be 10.4% (25) of the participants and candida was found in 6.5%. Highest resistance rate of E. coli growth was found in Co-trimoxazole (81%), Nalidixic acid (80 %), Ampicillin (78%), Ciprofloxacin (70%) and levofloxacin (60%). It was found 32.26% of participants who are having Levofloxacin-resistant Escherichia coli with 32µg/ml Minimum Inhibitory concentration (MIC). 40.91% of participants showed Ciprofloxacin resistant Escherichia coli with 64µg/ml MIC. Conclusion: In the present study, 62.2% of E.coli growth in their culture who was complained as urinary tract infection. The empirical guidelines of UTI treatments and prophylactics that are optimized against uropathogens without altering the normal development of microflora.
Abstract (English)
Background: Antimicrobial resistance among Escherichia coli causing urinary tract infection (UTI) is a public health concern. Objectives: 1. To find the prevalence of E. coli causing urinary tract infections from urine samples and to determine the antimicrobial susceptibility pattern of E. coli isolates by Kirby Bauer’s disc diffusion method. 2. To study about the minimum inhibitory concentration (MIC) fluoroquinolone resistant E. coli isolates by Microbroth dilution method. Method: The Prospective study was conducted among 150 UTI Patients attending Tertiary care centre for treatment. Ethical principles were adhered. Their socio – demographic details were collected and received urine samples were processed using standard methods and antibiotic susceptibility was done by Kirby-Bauer disk diffusion test and minimum inhibitory concentration of fluoroquinolone resistant E. coli isolates by Microbroth dilution method. Data was analysed using SPSS Software version 23.0. Results: The mean age of the participants was 48.55 + 14.37. Females were predominant. Majority, 49.3% belonged to 31-60 years age group. Among the 241 isolated organisms from culture, the most common were E. coli 62.2% (150) and Klebsiella pneumoniae was found to be 10.4% (25) of the participants and candida was found in 6.5%. Highest resistance rate of E. coli growth was found in Co-trimoxazole (81%), Nalidixic acid (80 %), Ampicillin (78%), Ciprofloxacin (70%) and levofloxacin (60%). It was found 32.26% of participants who are having Levofloxacin-resistant Escherichia coli with 32µg/ml Minimum Inhibitory concentration (MIC). 40.91% of participants showed Ciprofloxacin resistant Escherichia coli with 64µg/ml MIC. Conclusion: In the present study, 62.2% of E.coli growth in their culture who was complained as urinary tract infection. The empirical guidelines of UTI treatments and prophylactics that are optimized against uropathogens without altering the normal development of microflora.
Files
IJPCR,Vol16,Issue3,Article38.pdf
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Additional details
Dates
- Accepted
-
2024-02-26
Software
- Repository URL
- http://impactfactor.org/PDF/IJPCR/16/IJPCR,Vol16,Issue3,Article38.pdf
- Development Status
- Active
References
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