ASSESSMENT OF IN VITRO ANTIOXIDANT ACTIVTY OF HYDRO ALCOHOLIC EXTRACT OF SPATHODEA CAMPANULATA P.BEAUV

Antioxidant nature of SpathodeaCampanulata P. Beauv leaves is documented in ancient texts of Ayurveda and is used for symptomatic relief of neurological disorders along with urogenital disorders, analgesic and anti-inflammatory. The diversified activity arises from antioxidant phytochemicals like polyphenols, tannins and flavonoids. The evaluation of antioxidant property was formed in vitro by 2,2-diphenyl-1-picryl hydrazyl radical (DPPH) assay and was compared with ascorbic acid as reference standard. Ferric reducing power of the extract was also evaluated by using potassium ferricyanide. Free radical scavenging activity of the extract is in the range of 1.34 & 56.34 percentage inhibition, compared to ascorbic acid (2.32% and 64.00%) in reducing power assay. In DPPH, antioxidant evaluation of extract (23.32 % and 76.00 %) and ascorbic acid (46.21% and 86.45%). In conclusion, the dose dependent property observed may be useful in the formulation of the extract.