Pan-cancer profiling of tumor-infiltrating natural killer cells through transcriptional reference mapping

We generated a comprehensive reference atlas of human NK cells, based on single-cell RNA sequencing profiles from 72,129 cells obtained from bulk (12 donors) and sorted phenotypically-defined NK cell subsets (2 donors) and tissue-resident NK cells (TrNK, 136 donors). 38,982 tumor-infiltrating NK cells (TiNK) were extracted from datasets comprising seven solid tumor types (427 patients) and incorporated into our reference map using transfer learning. Both the NK data as well as the data form tumors/tissues with all the other cell types can be found here.