Adult neurogenesis improves spatial information encoding in the mouse hippocampus - hM4Di Silencing

In vivo two-photon imaging dataset for Frechou et al. "Adult neurogenesis improves spatial information encoding in the mouse hippocampus"

This dataset includes data from CNO-induced chemogenetic silencing experiments in hM4Di+ and hM4Di-(control) mice. There is another Zenodo dataset with the remaining hM4Di-(control) mice here. The folder for each mouse includes baseline (pre) and post-CNO (cno) recordings.

For each recording we included raw imaging data consisting of:

• Individual frames (.tif files) from 3 consecutive 3 min Ca2+ imaging movies (which were concatenated for analysis)
• Microscope settings metadata (Experiment.xml)
• Mouse location data (Episode001.h5 in SyncData folder) containing rotary encoder and RFID data

Some analyzed data is also included:

• Suite2p analysis data (Suite2p folder)
• fluorescence.npy contains raw fluorescence data (the F output of Suite2p data extraction). Rows are individual cells and columns are frames (i.e. timepoints) acquired at 15.253 Hz.
• positions.npy contains the position of the mouse on the treadmill belt indexed from 0 to 100.

Both NumPy(.npy) files are the output of the Preprocessing.py code, part of the analysis pipeline used for data analysis in the original publication, which can be found at https://github.com/GoncalvesLab/Frechou-et-al-Neurogenesis

Refer to the original publication for additional information. The sex of individual mice is as follows: