Positive autoregulation of Sox17 is necessary for gallbladder and extrahepatic biliary duct formation

This is a processed data (Seurat object) that contain single cell RNA-seq data from mouse , E10.5, Sox17∆50/∆50 mutant and Sox17+/+ embryos enriched for EPCAM+/CXCR4+ endodermal cells. Dataset contains 2912 total cells post qc. Sox17+/+ and Sox17Δ50/Δ50 cell samples are labelled with barcodes from 3'CellPlex Kit (10X Genomics). CellPlex Barcode ID CMO307 represent Sox17+/+ and CMO309 represent Sox17Δ50/Δ50 cells (included in assay named "CMO"). Genotype labels are under metadata column "genotype" as well. Libraries were constructed according to the manufacturer’s instructions for 10x Genomics Single Cell 3′ Library  kit v3. Demultiplex, qc and alignment was performed using 10X Genomics Cell Ranger software v3.0.2 and mm10 genome version index from 10X genomics(https://cf.10xgenomics.com/supp/cell-exp/refdata-gex-mm10-2020-A.tar.gz) was used. Additional quality control filtration, normalization, unsupervised clusteringwas performed using Seurat v4 R package.

The raw data will be available in Biostudies (https://www.ebi.ac.uk/biostudies/) when published under accession ID E-MTAB-14000.