Supplemenary Materials for manuscript "Accumulation dynamics of defective genomes during experi-mental evolution of two betacoronaviruses" by Hillung J., Olmo-Uceda, M.J., Muñoz-Sánchez, J.C., Elena, S.F.

Figure S1: Percentage of sgRNAs over the total number of deletions observed per sample. Thick lines are only shown for illustrating the average trend over time

Figure S2: Distribution of deletion type DVGs at low MOI. To facilitate visualization only deletions bigger than 50 nucleotides are shown. The number represent the exact length of the DVG and in parenthesis the lineages where they were founded.

Table S1: Statistics describing the variation in MOI for each experimental evolution lineage. Median values ±IQR are shown. Last column shows the fold difference between high and low MOIs (averaging lineages).

Table S2: Fitting of viral load data to the best-fitting ARIMA(p, d, q) model based on the minimum BIC criterium. Errors represent ±1 SE.