FLOURIMETRY – REVIEW ARTICLE

Flourimetry is a measurement of fluorescence intensity at a particular wavelength involving the phenomena of emission of radiation when there is transition from singlet excited state to singlet ground state. The wavelength of absorbed radiation is called as excitation wavelength and that of emitted radiation is called as emission wavelength. Phosphorescence is a specific type of photo luminescence related to fluorescence. In fluorescence, the molecules of the substance from the ground state excite to singlet state and return back to the ground state. In phosphorescence molecules of the substance in triplet excited state return to ground state. The deuterium lamp was used as a source of light, primary and secondary filter are built in it, quartzs,cuvettes was used for handling the sample come photo multiplier tube was employed as the detector LED (Fluorimeter model LF-2) (QUANTALASE India). The percentage fluorescence intensity was determined. The results were analysed for precision, accuracy, specificity, robustness and ANOVA test was performed. It gave accurate results in microgram concentration in solution.

Keywords: Flourescence, phosphorescence, deuterium lamp, photomultiplier tube, ANOVA,