Published March 13, 2024 | Version v1
Journal article Open

Antibody Characterization Report for FIG4

  • 1. ROR icon Montreal Neurological Institute and Hospital
  • 2. Advanced BioImaging Facility (ABIF)
  • 3. ROR icon McGill University
  • 4. The Neuro's Early Drug Discovery Unit (EDDU)

Description

This report presents a guide to selecting high-quality commercial antibodies against FIG4 (Polyphosphoinositide phosphatase)by Western blot, immunoprecipitation and immunofluorescence, using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls.

This study was funded in part by the ALS-Reproducible Antibody Platform (ALS-RAP), established as a public-private partnership by three prominent ALS charities - the ALS Association (USA), the Motor Neurone Disease Association (UK), and the ALS Society of Canada.

Files

FIG4_Polyphosphoinositide phosphatase_YCharOS report.pdf

Files (2.1 MB)

Additional details

Dates

Submitted
2024-03-13