GAS CHROMATOGRAPHY -ARTICLE REVIEW

Gas chromatography (GC) is an important technique used for the analysis of mixture. In these instruments (GC Digital Gas Flow Meter) (GC-2010 Pro) the method allows mixtures to separate into each component and determine the amounts of components present in sample. By using Gas chromatography, we can analyze a very small amount (microliters) of sample. The sample to be analyzed by GC must be volatile and thermally stable. Helium gas is used to carry the vaporized sample, where the components of sample flow with different rates. The vaporized sample is allowed to flow in along tube having a porous material called column. As column temperature raised, vapour pressure analyte increases eluted faster. The flame ionization detector is used to analyses the parameters like retention time, retention volume, separation factor, Area under the curve (AUC). The analytical process is relatively fast, accurate and precise.

Keywords: Gas chromatography, Method development, Columns, Volatile, thermostable, Flame ionization detector, Accuracy, Retention time, Retention volume, separation factor, Area under the curve, Derivatization.