Neogreenia sophorica Wu in Wu & Cheng, 2006

Neogreenia sophorica Wu in Wu & Cheng 2006: 62. Type data: CHINA: Beijing, Dongcheng District, on Sophora japonica. Holotype, female, type depository: Beijing Forestry University, Beijing, China.

Host plants: Styphnolobium japonicum (L.) Schott (Fabaceae).

Distribution: China (Beijing).

Remarks. Wu & Cheng (2006) described and illustrated the adult female and first-instar and second-instar nymphs. However, the description of the second-instar nymph of N. sophorica was based on specimens mixed with third-instar female nymphs. After examining these cysts, we noticed that the second-instar nymphs had (i) far fewer large simple pores and (ii) the five anterior abdominal spiracles each had sieve-like disc-pores in the atrium, whereas the third-instar female nymphs had (i) groups of large simple pores on the dorsum and (ii) the six anterior abdominal spiracles each had sieve-like disc-pores in the atrium. We therefore describe the second-instar and third-instar female nymphs of N. sophorica below.

Material examined. Paratypes: 3 second-instar nymphs, CHINA: collection data same as for holotype, mounted together on 1 slide; 6 third-instar ♀♀ nymphs, collection data same as for holotype, 24.iv.2004, mounted together on 1 slide.

Second-instar nymph (cyst) (sexes indistinguishable)

Slide-mounted material (n=3) (Fig. 6). Body oval, 1.0– 1.6 mm long and 0.6–0.9 mm wide; derm entirely membranous or with posterior part slightly sclerotized. Antennae (Fig. 6A) reduced and plate-like, each 10–13 μm in diameter, with margin sclerotized and protruding anteriorly, bearing 6 or 7 setae, each 15–25 μm long. Eyes absent. Labium (Fig. 6B) 2 segmented, about 80 μm long and 68–80 μm wide; segment I with a pair of short setae (each about 5 μm long) on each side; segment II with 2 apical hair-like setae (each about 23 μm long) on each side. Clypeolabral shield longer than labium, 235–260 μm long and 130–150 μm wide; stylets present. Legs absent. Thoracic spiracles (Fig. 6C) sclerotized, each opening about 10 μm in diameter, with a sclerotized bar and a group of 5 sieve-like disc-pores (Fig. 6D) in atrium. Abdominal spiracles numbering 8 pairs, with 1 pair situated on each abdominal segment, anterior 5 pairs sclerotized; anteriormost pair (Fig. 6E) with each opening about 10 μm in diameter, and 2 or 3 sieve-like disc-pores (Fig. 6D) within atrium; spiracles (Fig. 6F) on segments II– V smaller, each with opening about 7.5 μm in diameter, and with only 1 sieve-like disc-pore (Fig. 6D) within atrium; posteriormost 3 pairs unsclerotized and tube-like, with each opening about 2.5 μm in diameter, and lacking pores within atrium. Anal opening about 10 μm wide, with U-shaped sclerotized partial ring lacking pores and setae, located medially on posteriormost dorsal segment. Cicatrices absent.

Dorsum. With disc-pores of 2 types: (i) large simple pores (Fig. 6G), each about 10 µm in diameter, with sclerotized rim, numbering 0–2 pores (mostly 1) submarginally on each segment; (ii) compound multilocular disc-pores (Fig. 6H), each about 7.5 µm in diameter, with 3–7 (mostly 4 or 5) subcentral loculi and with outer ring of distinct peripheral loculi, present on margins from head to abdominal segment III. Setae of only 1 type: spine-like setae (Fig. 6I), each 6–8 μm long, present in submarginal and submedial areas.

Venter. With disc-pores of 3 main types: (i) compound multilocular disc-pores (Fig. 6H), same size and structure as on dorsum; forming group around each thoracic spiracle and present on margins except for 2 posteriormost segments; also smaller ones (Fig. 6J), each about 6 µm in diameter, with 3–5 subcentral loculi, numbering 2 or 3 between antennae; (ii) sieve-like disc-pores (Fig. 6D), each about 5 µm in diameter, with many irregularly distributed loculi, present within atria of thoracic spiracles and anterior 5 pairs of abdominal spiracles; and (iii) large simple pores (Fig. 6G), same size and structure as on dorsum; with 1 pore near each long hair-like seta on prothorax. Setae of 3 types present: (i) hair-like setae, each 18–28 μm long, situated submedially on prothorax, below each mesothoracic spiracle and submarginally on abdominal segment I; (ii) spine-like setae (Fig. 6I), same size as on dorsum, a few on margins; and (iii) short conical spine-like setae (Fig. 6K) with sclerotized basal sockets, each about 5 μm long; with 1 or 2 near each hair-like seta, 2 or 3 near each thoracic spiracle and 0 or 1 near each abdominal spiracle except for posteriormost pair, where absent.

Third-instar female nymph (cyst)

Slide-mounted material (n=6) (Fig. 7). Body pyriform, rounded anteriorly and tapering posteriorly, 2.8–3.9 mm long and 1.8–2.0 mm wide; derm mostly membranous but abdominal apex sclerotized. Antennae (Fig. 7A) reduced to small oval plates, each 20–25 μm in diameter, with margin sclerotized and protruding anteriorly, bearing 5 or 6 setae, each 12–40 μm long; situated medially on head. Eyes absent. Labium (Fig. 7B) 2 segmented, 130–150 μm long and 100–110 μm wide; segment I 53–65 μm long, with margins sclerotized but centre membranous, bearing 1 pair of short setae (each 30–33 μm long) on each side; segment II 78–85 μm long, sclerotized, with 3 apical hair-like setae (each 20–25 μm long) on each side. Clypeolabral shield longer than labium, 370–385 μm long and 160–190 μm wide; stylets present. Legs absent. Thoracic spiracles (Fig. 7C) sclerotized, each opening 12–18 μm in diameter, with sclerotized bar and group of 12–14 sieve-like disc-pores (Fig. 7D) at inner end of atrium. Abdominal spiracles numbering 8 pairs, with anterior 6 pairs sclerotized; 5 anteriormost pairs (Fig. 7E) each with opening about 20 μm in diameter and 4–7 sieve-like disc-pores in atrium; spiracles (Fig. 7F) on abdominal segment VI much smaller, each with opening 9–10 μm in diameter and atrium containing 2 sieve-like disc-pores (Fig. 7D); posteriormost 2 pairs of spiracles unsclerotized, small and tube-like, each with opening about 5 μm in diameter, lacking pores within atrium. Anal opening with U-shaped sclerotized ring, 18–20 μm wide, without pores or setae, located medially on posteriormost dorsal segment. Cicatrices absent.

Dorsum. With disc-pores of 2 types: (i) large simple pores (Fig. 7G), each 13–15 µm in diameter, with sclerotized rim, forming group of 3–20 pores on submargin of each segment, most numerous on prothorax; (ii) compound multilocular disc-pores (Fig. 7H), each about 10 µm in diameter, with 4–6 subcentral loculi and an outer ring of 12–14 peripheral loculi, present on margins from head to abdominal segment II. Setae of only 1 type present: spine-like setae (Fig. 7I), each 8–10 μm long, present in submarginal and marginal areas.

Venter. With disc-pores of 4 main types: (i) large simple pores (Fig. 7G), same size and structure as on dorsum, numbering 1–4 near each hair-like seta on prothorax; (ii) compound multilocular disc-pores (Fig. 7H), same size and structure as on dorsum; forming group around each thoracic spiracle and present on margins except absent from 2 posterior abdominal segments; also smaller ones (Fig. 7J), each 8–9 μm in diameter, with 5 subcentral loculi and 4–6 peripheral loculi irregularly distributed, these smaller pores numbering 5–7 between antennae; and (iv) sieve-like disc-pores (Fig. 7D), each about 7.5 µm in diameter, some pores slightly polygonal, each with many irregularly distributed loculi, present within atria of thoracic spiracles and anterior 6 pairs of abdominal spiracles. Setae of 3 types: (i) hair-like setae, each 23–25 μm long, situated submedially on prothorax, and submarginally on mesothorax and abdominal segment I; (ii) spine-like setae (Fig. 7I), same size as on dorsum, few on margin; and (iii) short conical spine-like setae (Fig. 7K) with sclerotized basal sockets, each about 5 μm long; with 1 or 2 near each hair-like seta, 3–5 around each thoracic spiracle and 1–4 around each abdominal spiracle.