Published March 1, 2024 | Version v1
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Videos for PolNet GUI

  • 1. Universidad de Murcia

Description

Contains videos to show usage examples of the different scripts of the PolNet GUI. Specifically, for constructing all the input models necesary generating a single synthetic tomogram (400x400x100 voxels with 10 Angstron voxel size) with two different cytosolic macromolecules, a membrane bound one, microtubules and membranes with ellipsoidal shape.

  1. create_membrane_models: generates the configuration file of structural model for membranes.
  2. create_membrane_models: generates the configuration file of structural model for filaments.
  3. atomic_to_density: converts PDB atomic models into density map models for macromolecules.
  4. align_membrane_proteins: aligns a density map of a protein with respect to a reference membrane, required for membrane bound proteins.
  5. create_macromolecule_models: generates the configuration file of structural model for macromolecules, inclusing membrane bound ones.
  6. gen_dataset_and_results: shows a example of selecting the input files and parameters to generate a single synthetic tomogram, it also contain examples of how to visualize the output files.

Files

align_membrane_proteins.mp4

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Additional details

Related works

Is supplement to
Preprint: 10.1101/2023.05.26.542411 (DOI)
Software: https://github.com/anmartinezs/polnet.git (URL)