Supplementary videos for PhD Thesis: Development of a high resolution light sheet microscope utilising remote refocussing

Supplementary videos for PhD Thesis: Development of a high resolution light sheet microscope utilising remote refocussing

The included videos are the following:

1. An illustration of the unintuitive viewing perspective presented by OPM.
2. Example operation of the live deskewing software operated in global update mode.
3. Example operation of the live deskewing software using the shear warp algorithm to render rotated perspectives of the sample.
4. Example operation of the live deskewing software operated in rolling update mode.
5. COS-7 cell endoplasmic reticulum restructuring imaged at 5 VPS.
6. COS-7 cell mitochondria dynamics imaged at 0.9 VPS.
7. Random motion of GUVs in solution imaged at a rate of a volume every 6.2s.
8. Degradation of the inner domain of enzyme responsive DNA condensates after the addition of UDG imaged at a rate of 1 volume every 2 minutes.
9. Degradation of the outer domain of enzyme responsive DNA condensates after the addition of RNaseH imaged at a rate of 1 volume per minute. 
10. Degradation of the outer domain of enzyme responsive DNA condensates after the addition of RNase H as a function of depth in the condensate. 
11. Degradation of the inner domain of enzyme responsive DNA condensates after the addition of UDG as a function of depth in the condensate. 
12. A 5.5 hour timelapse of PANC-1 cells incubated with human serum albumin-based nanoparticles imaged at 1 volume every 10 minutes.
13. A 40 minute timelapse of PANC-1 cells post addition of human serum albumin-based nanoparticles, imaged at 1 volume every 20 seconds.