RP - HPLC Analytical Method Development and Validation for the Simultaneous Estimation of Cabotegravir and Rilpivirine In Bulk and Tablet Dosage Form

ABSTRACT

A simple, Accurate, precise method was developed for the simultaneous estimation of the Rilpivirine and Cabotegravir in dosage form. Chromatogram was run through AgilentC18 150 x 4.6mm, 5.0mm. ⁽¹⁾ Mobile phase containing Buffer 0.1% Ammonium Acetate: Acetonitrile taken in the ratio 55:45v/v was pumped through the column at a flow rate of 1.0 ml/min. Temperature was maintained at 30°C. The optimized wavelength selected was 260 nm. The retention time of Rilpivirine and Cabotegravir was found to be 2.238 min and 2.953 min. %RSD of the Rilpivirine and Cabotegravir was found to be 0.5 and 0.5 respectively. %Recovery was obtained as 99.85% and 99.84% for Rilpivirine and Cabotegravir respectively. LOD and LOQ values obtained from regression equations of Rilpivirine and Cabotegravir were 0.11, 0.3,2, and 0.02, 0.06 respectively. ⁽²⁾ The regression equation of Rilpivirine is y = 17712x + 2324.3 and y = 17293x + 1410.5 of Cabotegravir Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.  

Keywords: Rilpivirine, Cabotegravir, RP-HPLC, Validation.