Studies on Phosphate Solubilizing Microorganisms Isolated From Soil

In the present study phosphates solubilizing microbial (PSM) cultures were isolated from the collected soil samples. Rhizospheric microflora such as Trichoderma, Aspergillus, Penicillium, Rhizobium, Pseudomonas, Azotobacter sp. were isolated as a phosphate solubilizing strains using selective agar media. The isolated bacterial cultures were recognized by culture dependent method and the fungal species were identified by morphological characters. The bacterial isolates were incubated at 35°C for 24-48 hrs in incubator while fungal cultures were incubated at 35°C for 48-72 hrs in incubator. The serial dilution as 10^-4, 10^-5, 10^-6 and 10^-7 were preferred for the isolation of PSM. On Pikovaskays agar plate, the zone of clearance verified phosphate solubilization. The identified strains may be useful in enhancing crop yield, preventing plant diseases, and promoting growth.