Published February 18, 2024 | Version v1
Journal article Open

MeIS: DNA methylation-based immune response signatures for thyroid nodule diagnostics

  • 1. Department of Pathology, China-Japan Friendship Hospital, Beijing, China
  • 2. Singlera Genomics (Shanghai) Ltd., Shanghai, 201203, China
  • 3. Department of Interventional Radiology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China
  • 4. National Clinical Research Center for Interventional Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200030, China
  • 5. Shanghai Institute of Medical Imaging, Fudan University, Shanghai, 200032, China

Description

Supplementary Materials

Supplementary Table 1. Genomic positions of the fifteen markers.

Supplementary Table 2. MeIS diagnostics for Yim's Cohort.

Supplementary Table 3. Fifteen markers diagnostics of thyroid nodules in Timp's dataset by LOOCV.

Supplementary Table 4. Fifteen markers diagnostics of thyroid nodules in Reis’s dataset by LOOCV.

Supplementary Table 5. Comparing MeIS performance in patients with chronic lymphocytic thyroiditis versus those without.

Supplementary Table 6. MeIS diagnostics for the ZS's retrospective cohort.

Supplementary Table 7. MeIS diagnostics for the ZS's preoperative cohort.

Supplementary Table 8. Comb diagnostics for cytologically indeterminate samples in Yim's Cohort.

Supplementary Table 9. Comb diagnostics for cytologically indeterminate samples in ZS's retrospective Cohort.

Supplementary Table 10. Comb diagnostics for cytologically indeterminate samples in ZS's preoperative Cohort.

Supplementary Figure 1. NMF-based deconvolution of the methylome in thyroid nodules. A. Plot of cophenetic correlation coefficients. B. Heatmap of consensus clustering of samples by three components.

Supplementary Figure 2. ImmunoScores in the two cohorts. A. ImmunoScores in the training cohorts. B. ImmunoScores in the validation cohorts. The Mann–Whitney U test was used to compare ImmunoScores between PTCs and BTNs.

Supplementary Figure 3. Boxplots of MeIS predictive scores by different clinical factors in the validation cohort. A. Age. B. Sex. C. Metastasis. D. Thyroiditis. E. BRAF V600E mutation. The Mann–Whitney U test was used to compare scores between two groups.

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