Multiplexed spatial profiling of Hodgkin Reed-Sternberg cell neighborhoods in classic Hodgkin lymphoma

Published February 15, 2024 | Version v3

Dataset Restricted

Single-cell data from multiplexed imaging (Leica Cell DIVE) of 29 proteins in 587 fields of view across 30 classic Hodgkin lymphoma patients.

Bulk RNA sequencing (NanoString IO360 panel) raw and normalized data for 32 classic Hodgkin lymphoma patients.

Meta data containing information about patients, cell type and state information, and statistical comparisons.

Table of contents (English)

Individual file descriptions

RawData.csv
- Raw NanoString data (32 patients)
NormaizedData.csv
- Normalized NanoString data (32 patients)
Files beginning with HL_*
- Normallized single-cell mpIF data (30 patients, 1 per file)
HodgkinLymphoma_Samples.xlsx
- Meta data containing patient-level information
HodgkinLymphoma_FOVs.xlsx
- Meta data containing field of view-level information (mpIF)
HodgkinLymphoma_Markers.xlsx
- Meta data containing information on markers in mpIF panel
HodgkinLymphoma_CellTypes.xlsx
- Meta data containing cell type definitions (mpIF)
HodgkinLymphoma_CellStates.xlsx
- Meta data containing cell state information (mpIF)
HodgkinLymphoma_Questions.xlsx
- Meta data containing list of statistical comparisons (mpIF)
nanostring_CIBERSORT.csv
- CIBERSORT results
2016_Newman_NatMethods_PMID_25822800_lm22.csv
- List of LM22 genes (PMID: 25822800)
annotate_gene_sets_nanostring.xlsx
- List of genesets and corresponding genes annotated in heatmap
mpif_celltype_counts.csv
- mpIF cell type counts
HRS_agpres.csv
- Coexpression of B2M/MHCI/MHCII in HRS cells (mpIF)
hrscov.xlsx
hrscov_order.csv
- Coefficient of variation for all HRS cells states (mpIF)
hrsagg.xlsx
hrsagg_detail.xlsx
- HRS aggregate data (mpIF)

*Note: all files are formatted for use with the various pipelines

The record is publicly accessible, but files are restricted to users with access.