Demo 10x Visium dataset for STQ
Description
The small dataset includes FASTQ files with 13,043,592 reads from 65 Visium spots of sample WM4237_TE_S1_ST. See https://doi.org/10.1101/2023.07.27.550727 for the full datasets details. Below is the code used to subset FASTQ reads to the 65 spots selected in "subset.csv"
fastq1="SC2200092_WM4237-1-1337/fastq/SC2200092_GT22-04384_AGTTTCCTGG-CTGTGTGGCA_S2_L001_R1_001.fastq"
fastq2="SC2200092_WM4237-1-1337/fastq/SC2200092_GT22-04384_AGTTTCCTGG-CTGTGTGGCA_S2_L001_R2_001.fastq"fgrep -B 1 -f subset.csv ${fastq1} | grep "@" | cut -d' ' -f1 > readnames.list
seqtk subseq ${fastq1} <(cat readnames.list | cut -d '@' -f2) > subset_L001_R1_001.fastq
seqtk subseq ${fastq2} <(cat readnames.list | cut -d '@' -f2) > subset_L001_R2_001.fastqgzip -kv subset_L001_R1_001.fastq
gzip -kv subset_L001_R2_001.fastq
The data includes output of STQ demo run.
Files
SC2200092.tiff
Additional details
Related works
- Is source of
- Preprint: 37546876 (PMID)
Software
- Repository URL
- https://github.com/TheJacksonLaboratory/STQ
- Programming language
- Nextflow , Python
- Development Status
- Active