A REVIEW ON METHOD DEVELOPMENT AND VALIDATION OF DECITABINE BY DIFFERENT ANALYTICAL TECHNIQUES

Pharmaceutical analysis is a broader term which can be defined in many ways. It is the series of processes that are used for identification, determination, separation, purification, and structure elucidation of the given compound used in the formulation of pharmaceutical products. Recent development in analytical methods has been resulted from the advancement of analytical instruments. The improvement of the analytical method development and analytical instruments like UV, HPLC, LC-MS, GC are required for reduced the time of analysis, increased precision and accuracy and reduced costs of analysis. Decitabine (i.e., 5-aza-2′-deoxycytidine) acts as a nucleic acid synthesis inhibitor.It is a medication for the treatment of myelodysplastic syndromes, a class of conditions where certain blood cells are dysfunctional, and for acute myeloid leukemia (AML). Chemically, it is a cytidine analog.

Key words: Analytical method development, validation, Decitabine, UV, HPLC, LC-MS, GC