Published February 28, 2019 | Version v1
Taxonomic treatment Open

Peucedanum praeruptorum Dunn

Description

2.1. Identification of the cDNA encoding PAL in P. praeruptorum

Using PAL sequences reported previously and our transcriptome database of P. praeruptorum (accession no. SRX997427) (Zhao et al., 2015), a BLAST search was conducted. According to the E -value of alignments, only one PAL was identified and cloned. This cDNA, which encodes a 719 amino acid residue protein (Table S1), was designated PpPAL. According to bioinformatics, PpPAL is most closely related to PAL of Angelica sinensis (AsPAL), which also belongs to Apiaceae (Fig. S2). The amino acid sequences of PpPAL also contain a highly conserved MIO group, which is formed by Ala-Ser-Gly residues (Fig. S3). The high sequence similarity revealed that they might display the same enzymatic functions such as substrate specificity, optimal pH and catalytic mechanism.

Notes

Published as part of Sui, Ziwei, Luo, Jun, Yao, Ruolan, Huang, Chuanlong, Zhao, Yucheng & Kong, Lingyi, 2019, Functional characterization and correlation analysis of phenylalanine ammonia-lyase (PAL) in coumarin biosynthesis from Peucedanum praeruptorum Dunn, pp. 35-45 in Phytochemistry 158 on page 36, DOI: 10.1016/j.phytochem.2018.11.006, http://zenodo.org/record/10481556

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Additional details

Biodiversity

Scientific name authorship
Dunn
Kingdom
Plantae
Phylum
Tracheophyta
Order
Apiales
Family
Apiaceae
Genus
Peucedanum
Species
praeruptorum
Taxon rank
species

References

  • Zhao, Y. C., Liu, T. T., Luo, J., Zhang, Q., Xu, S., Han, C., Xu, J. F., Chen, M. H., Chen, Y. J., Kong, L. Y., 2015. Integration of a decrescent transcriptome and metabolomics dataset of Peucedanum praeruptorum to investigate the CYP 450 and MDR genes involved in coumarins biosynthesis and transport. Front. Plant Sci. 6, 996. https: // doi. org / 10. 3389 / fpls. 2015.00996.